Jurkat cells were subjected to SDS PAGE followed by western blot with 15660-1-AP(CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15660-1-AP(CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

1X10^6 SH-SY5Y cells were stained with 0.2ug CADM3 antibody (15660-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.