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CADM3 Polyclonal antibody

CADM3 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC

Conjugate

Unconjugated

Cat no : 15660-1-AP

Synonyms

BIgR, Brain immunoglobulin receptor, CADM3, cell adhesion molecule 3, IGSF4B, Necl 1, NECL1, Nectin like protein 1, synCAM3, TSLC1 like protein 1, TSLL1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CADM3 Polyclonal antibody (15660-1-AP)

    WB analysis using 15660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CADM3 Polyclonal antibody (15660-1-AP)

    WB analysis of SH-SY5Y using 15660-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CADM3 Polyclonal antibody (15660-1-AP)

    WB analysis of Jurkat using 15660-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CADM3 Polyclonal antibody (15660-1-AP)

    FC experiment of SH-SY5Y using 15660-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug CADM3 antibody (15660-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells, SH-SY5Y cells, mouse cerebellum tissue, rat brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15660-1-AP targets CADM3 in WB, FC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CADM3 fusion protein Ag8081
Full Name cell adhesion molecule 3
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38-50 kDa
GenBank Accession NumberBC033819
Gene Symbol CADM3
Gene ID (NCBI) 57863
RRIDAB_2068044
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CADM3, also known as IGSF4B or TSLL1, is a transmembrane protein that is involved in the cell-cell adhesion. CADM3 is mainly expressed in the nervous system and plays a role in the formation of synapses, axon bundles, myelinated axons and cerebellar morphogenesis (PMID: 18420026).

Protocols

Product Specific Protocols
WB protocol for CADM3 antibody 15660-1-APDownload protocol
FC protocol for CADM3 antibody 15660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols