CAMK2 Antibody

CAMK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13730-1-AP

Synonyms

CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CAMKA, CaMKII alpha, KIAA0968



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

13730-1-AP targets CAMK2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAMK2 fusion protein Ag4745
Full Name calcium/calmodulin-dependent protein kinase II alpha
Calculated molecular weight 478aa,54 kDa
Observed molecular weight 45-60 kDa
GenBank accession number BC040457
Gene symbol CAMK2A
Gene ID (NCBI) 815
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of CAMK2 family.

Protocols

Product Specific Protocols
WB protocol for CAMK2 antibody 13730-1-APDownload protocol
IHC protocol for CAMK2 antibody 13730-1-APDownload protocol
IF protocol for CAMK2 antibody 13730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Med Rep

Effects of intrathecal bupivacaine on the NR2B/CaMKIIα/CREB signaling pathway in the rat lumbar spinal cord.

Authors - Liyan Zhao
ratWB

J Mol Neurosci

Beneficial Effects of a CaMKIIα Inhibitor TatCN21 Peptide in Global Cerebral Ischemia.

Authors - Mohammad Ejaz Ahmed
ratWB

Exp Neurol

MicroRNA-153 impairs hippocampal synaptic vesicle trafficking via downregulation of synapsin I in rats following chronic cerebral hypoperfusion.

Authors - Shuai Zhang