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- KD/KO Validated
Caspase 8/p43/p18 Monoclonal antibody
Caspase 8/p43/p18 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (5)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2B9H8
Cat no : 66093-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 25 publications below |
| IF | See 3 publications below |
Product Information
The immunogen of 66093-1-Ig is Caspase 8/p43/p18 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | chicken, human, monkey, mouse, rat, sheep |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Caspase 8/p43/p18 fusion protein Ag20524 |
| Full Name | caspase 8, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 538 aa, 62 kDa |
| Observed molecular weight | 53-57 kDa, 32-45 kDa, 18 kDa |
| GenBank accession number | BC028223 |
| Gene symbol | CASP8 |
| Gene ID (NCBI) | 841 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 8/p43/p18 antibody 66093-1-Ig | Download protocol |
| IHC protocol for Caspase 8/p43/p18 antibody 66093-1-Ig | Download protocol |
| IF protocol for Caspase 8/p43/p18 antibody 66093-1-Ig | Download protocol |
| IP protocol for Caspase 8/p43/p18 antibody 66093-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Inflamm (Lond) Serp-2, a virus-derived apoptosis and inflammasome inhibitor, attenuates liver ischemia-reperfusion injury in mice. | ||
World J Gastrointest Oncol KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma. | ||
Hepatol Commun TRAIL Deletion Prevents Liver, but Not Adipose Tissue, Inflammation during Murine Diet-Induced Obesity. | ||
Int Immunopharmacol Oxyresveratrol prevents lipopolysaccharide/d-galactosamine-induced acute liver injury in mice. | ||
Int J Mol Med SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary. | ||
Cancer Chemother Pharmacol Wnt/β-catenin signaling mediates the suppressive effects of diallyl trisulfide on colorectal cancer stem cells. |
