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Caspase 8 Antibody 23 Publications

Mouse Monoclonal| Catalog number: 66093-1-Ig |CloneNo.: 2B9H8

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Con: 146 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Caspase 8 Antibody WB Jurkat cells 66093-1-Ig

Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

Caspase 8 Antibody WB HeLa cells 66093-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

Caspase 8 Antibody WB HeLa cells 66093-1-Ig

WB result of CASP8 with normal and apotosis Hela cell. P18 can be get in the apotosis cell. 60kd,50kd and 45kd bands are some isoforms of precursor CASP8.

Caspase 8 Antibody IP HepG2 cells 66093-1-Ig

IP Result of anti-Caspase 8 (IP:66093-1-Ig, 5ug; Detection:66093-1-Ig 1:500) with HepG2 cells lysate 2400ug.

Caspase 8 Antibody IHC human liver cancer tissue 66093-1-Ig

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig( Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caspase 8 Antibody IHC human liver cancer tissue 66093-1-Ig

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig( Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caspase 8 Antibody IF HeLa cells 66093-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 66093-1-Ig(Caspase 8 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, chicken, monkey, mouse, rat, sheep
Positive WB detected in:
Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human liver cancer tissue, human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Caspase 8 fusion protein Ag20524
Full name:
caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight:
538aa,62 kDa
Observed molecular weight:
53-57 kDa, 32-45 kDa, 18 kDa
GenBank accession number:
BC028223
Gene ID (NCBI):
841
Gene symbol
CASP8
Synonyms
Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5

Background

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.


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