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CoraLite®594-conjugated Caspase 8/p43/p18 Monoclonal antibody

Caspase 8/p43/p18 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b






CoraLite®594 Fluorescent Dye



Cat no : CL594-66093


ALPS2B, Apoptotic cysteine protease, Apoptotic protease Mch 5, CAP4, Casp 8, CASP8, Caspase 8, Caspase8, FADD like ICE, FLICE, FLJ17672, ICE like apoptotic protease 5, MACH, MCH5, MORT1 associated ced 3 homolog

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66093 is Caspase 8/p43/p18 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Caspase 8/p43/p18 fusion protein Ag20524
Full Name caspase 8, apoptosis-related cysteine peptidase
Calculated Molecular Weight 538 aa, 62 kDa
Observed Molecular Weight 53-57 kDa, 32-45 kDa, 18 kDa
GenBank Accession NumberBC028223
Gene Symbol CASP8
Gene ID (NCBI) 841
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.


Product Specific Protocols
IF protocol for CL594 Caspase 8/p43/p18 antibody CL594-66093Download protocol
Standard Protocols
Click here to view our Standard Protocols