Caspase 8/p43/p18 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Caspase 8/p43/p18 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B9H8

Cat no : 66093-1-PBS

Synonyms

Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5

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Validation Data Gallery

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  WB analysis of Jurkat using 66093-1-Ig (same clone as 66093-1-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa cells using 66093-1-Ig (same clone as 66093-1-PBS)

  WB result of CASP8 with normal and apotosis Hela cell. P18 can be get in the apotosis cell. 60kd,50kd and 45kd bands are some isoforms of precursor CASP8. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66093-1-Ig (same clone as 66093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66093-1-Ig (same clone as 66093-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (CASP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66093-1-Ig (same clone as 66093-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (CASP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 66093-1-Ig (same clone as 66093-1-PBS)

  IP result of anti-Caspase 8/p43/p18 (IP:66093-1-Ig, 5ug; Detection:66093-1-Ig 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (CASP8 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (CASP8 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66093-1-Ig(Caspase 8 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66093-1-PBS targets Caspase 8/p43/p18 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 8/p43/p18 fusion protein Ag20524
• Full Name: caspase 8, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 538 aa, 62 kDa
• Observed Molecular Weight: 53-57 kDa, 32-45 kDa, 18 kDa
• GenBank Accession Number: BC028223
• Gene Symbol: CASP8
• Gene ID (NCBI): 841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.