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CoraLite®488-conjugated Caspase 8/p43/p18 Monoclonal antibody

Caspase 8/p43/p18 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2B9H8

Cat no : CL488-66093

Synonyms

ALPS2B, Apoptotic cysteine protease, Apoptotic protease Mch 5, CAP4, Casp 8, CASP8, Caspase 8, Caspase8, FADD like ICE, FLICE, FLJ17672, ICE like apoptotic protease 5, MACH, MCH5, MORT1 associated ced 3 homolog



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66093-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66093 is Caspase 8/p43/p18 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Caspase 8/p43/p18 fusion protein Ag20524
Full Name caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight 538 aa, 62 kDa
Observed molecular weight 53-57 kDa, 32-45 kDa, 18 kDa
GenBank accession numberBC028223
Gene symbol CASP8
Gene ID (NCBI) 841
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.

Protocols

Product Specific Protocols
IF protocol for CL488 Caspase 8/p43/p18 antibody CL488-66093Download protocol
Standard Protocols
Click here to view our Standard Protocols