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Caveolin-1 Antibody 23 Publications

Rabbit Polyclonal| Catalog number: 16447-1-AP

Featured Product KD/KO validated

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Con: 38 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Caveolin-1 Antibody WB NIH/3T3 cells 16447-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16447-1-AP (Caveolin-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

Caveolin-1 Antibody WB HeLa cells 16447-1-AP

WB result of Caveolin-1 antibody (16447-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells

Caveolin-1 Antibody IHC human lung cancer tissue 16447-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caveolin-1 Antibody IHC human lung cancer tissue 16447-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16447-1-AP (Caveolin-1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caveolin-1 Antibody IP A549 cells 16447-1-AP

IP Result of anti-Caveolin-1 (IP:16447-1-AP, 3ug; Detection:16447-1-AP 1:500) with A549 cells lysate 1200ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
NIH/3T3 cells, A549 cells, HeLa cells
Positive IP detected in:
A549 cells
Positive IHC detected in:
human lung cancer tissue, human heart tissue, human liver tissue, human lung tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:600-1:5000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Caveolin-1 fusion protein Ag8049
Full name:
caveolin 1, caveolae protein, 22kDa
Calculated molecular weight:
22 kDa
Observed molecular weight:
20-25 kDa
GenBank accession number:
BC006432
Gene ID (NCBI):
857
Gene symbol
CAV1
Synonyms
BSCL3, CAV, CAV1, Caveolin 1, Caveolin-1, CGL3, MSTP085, VIP21

Background

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in astric Cancer (PMID: 25339030). MW of Caveolin-1 is from 20-25 kDa due to phosphorylation (PMID: 10198051).


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