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Caveolin-1 Polyclonal antibody
Caveolin-1 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 16447-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, NIH/3T3 cells |
Positive IP detected in | A549 cells |
Positive IHC detected in | human breast cancer tissue, human heart tissue, human lung cancer tissue, human liver tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 8 publications below |
WB | See 31 publications below |
IHC | See 8 publications below |
IF | See 7 publications below |
IP | See 1 publications below |
Product Information
The immunogen of 16447-1-AP is Caveolin-1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | bovine, human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Caveolin-1 fusion protein Ag8049 |
Full Name | caveolin 1, caveolae protein, 22kDa |
Calculated molecular weight | 22 kDa |
Observed molecular weight | 20-25 kDa |
GenBank accession number | BC006432 |
Gene symbol | CAV1 |
Gene ID (NCBI) | 857 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in astric Cancer (PMID: 25339030). MW of Caveolin-1 is from 20-25 kDa due to phosphorylation (PMID: 10198051).
Protocols
Product Specific Protocols | |
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WB protocol for Caveolin-1 antibody 16447-1-AP | Download protocol |
IHC protocol for Caveolin-1 antibody 16447-1-AP | Download protocol |
IP protocol for Caveolin-1 antibody 16447-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Am J Physiol Lung Cell Mol Physiol Identification of CAV1 and DCN as potential predictive biomarkers for lung adenocarcinoma. | ||
Am J Chin Med Puerarin Reduces Blood Pressure in Spontaneously Hypertensive Rats by Targeting eNOS. | ||
Biochem Pharmacol Betulinic acid suppresses breast cancer aerobic glycolysis via caveolin-1/NF-κB/c-Myc pathway. | ||
FASEB J Tumoral microvesicle-activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma.
| ||
Am J Respir Crit Care Med Distinct Cancer-Promoting Stromal Gene Expression Depending on Lung Function. | ||
EBioMedicine POH1 contributes to hyperactivation of TGF-β signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-β receptors and caveolin-1. |