CCR5 Polyclonal antibody

CCR5 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 17476-1-AP

Synonyms

C C chemokine receptor type 5, C C CKR 5, CC CKR 5, CCCKR5, CCR 5, CCR5, CD195, CHEMR13, CKR 5, CKR5, CMKBR5, HIV 1 fusion coreceptor, IDDM22



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17476-1-AP is CCR5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, Indian rhesus macaques
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCR5 fusion protein Ag11493
Full Name chemokine (C-C motif) receptor 5
Calculated molecular weight 352 aa, 41 kDa
GenBank accession numberBC038398
Gene symbol CCR5
Gene ID (NCBI) 1234
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCR5 (also known as CD195) is a member of the beta chemokine receptor family. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. The natural chemokine ligands that bind to CCR5 include MIP-1-alpha, MIP-1-beta, MCP-2 and RANTES.

Protocols

Product Specific Protocols
IHC protocol for CCR5 antibody 17476-1-APDownload protocol
FC protocol for CCR5 antibody 17476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
Indian rhesus macaquesIHC

J Neurovirol

Lack of susceptibility in neonatally infected rhesus macaques to simian immunodeficiency virus-induced encephalitis.

Authors - Elizabeth Delery
ratWB

Prog Neuropsychopharmacol Biol Psychiatry

PMID: 26190414

Authors - Klaudia Kwiatkowski
humanWB

Retrovirology

CCR5 editing by Staphylococcus aureus Cas9 in human primary CD4+ T cells and hematopoietic stem/progenitor cells promotes HIV-1 resistance and CD4+ T cell enrichment in humanized mice.

Authors - Qiaoqiao Xiao
human

J Biol Chem

Regulation of C-C chemokine receptor 5 (CCR5) stability by Lys197 and by transmembrane protein aptamers that target it for lysosomal degradation.

Authors - Lisa M Petti