CD1A Monoclonal antibody

CD1A Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H7B2

Cat no : 60173-3-Ig

Synonyms

CD1, CD1A, CD1a molecule, FCB6, HTA1, hTa1 thymocyte antigen, R4, T6



Tested Applications

Positive WB detected inpig thymus tissue, Jurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60173-3-Ig targets CD1A in WB, FC, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD1A fusion protein Ag11304
Full Name CD1a molecule
Calculated Molecular Weight 327 aa, 37 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC031645
Gene Symbol CD1A
Gene ID (NCBI) 909
RRIDAB_2881353
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.

Protocols

Product Specific Protocols
WB protocol for CD1A antibody 60173-3-IgDownload protocol
FC protocol for CD1A antibody 60173-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols