CD68 Polyclonal antibody

CD68 Polyclonal Antibody for FC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, FC, , ELISA

Conjugate

Unconjugated

Cat no : 25747-1-AP

Synonyms



Tested Applications

Positive IP detected inRAW 264.7 cells
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25747-1-AP is CD68 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD68 fusion protein Ag22815
Full Name CD68 molecule
Calculated molecular weight 37 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC015557
Gene symbol CD68
Gene ID (NCBI) 968
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.

Protocols

Product Specific Protocols
IP protocol for CD68 antibody 25747-1-APDownload protocol
FC protocol for CD68 antibody 25747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hum Reprod

Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.

Authors - Yanling Gou
humanWB

Toxicol Appl Pharmacol

Multi-walled carbon nanotubes promoted lipid accumulation in human aortic smooth muscle cells.

Authors - Haiyin Yang
humanIHC

Cancer Lett

PMID: 31055110

Authors - Xiaohang Chen
humanIHC

Am J Neurodegener Dis

Neuronal phagocytosis by inflammatory macrophages in ALS spinal cord: inhibition of inflammation by resolvin D1.

Authors - Liu Guanghao G
mouseIF

J Cardiovasc Pharmacol Ther

PMID: 25818930

Authors - Mingxue Zhou
humanIF

Data Brief

Data supporting attempted caveolae-mediated phagocytosis of surface-fixed micro-pillars by human osteoblasts.

Authors - Caroline Moerke

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Fabio Henrique (Verified Customer) (01-17-2019)

Figure 1. IF of CD68 (red) in human spleen. Formalin-fixed paraffin-embedded human spleen tissue was used as positive control to probe for CD68. Heat-induced antigen retrieval was performed in sodium citrate buffer pH 6.0 + Tween20 at 0.5%. Tissue incubated at 96*C for 20 min inside the buffer. Permeabilization was done with washes of TBSTritonX 0.25% for 3x 5min and blocking was done in TBS 10% Goat serum, 1% BSA, for 1 hr at RT. Anti-CD68 was used at 1:200 in blocking buffer, overnight incubation at 4*C. Secondary antibody AlexaFluor 488 was used at 1:500 for 1 hr at RT. Imaged using a Nikon A1 confocal microscope. Figure 2: CD68 (red) and CD206 (green) in human primary macrophages polarized to M2-phenotype, encapsulated in 3D hydrogel (hyaluronic acid and collagen type 1). Staining was performed as described above, except primary (CD68) was used at 1:100 and secondary antibodies were used at 1:500.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100 (hydrogels); 1:200 (2D samples)
  • Cell Tissue Type: Spleen and 3D culture of human primary macrophages
CD68 Antibody Immunofluorescence, validation (1:100 (hydrogels); 1:200 (2D samples) dilution) in Spleen and 3D culture of human primary macrophages (Cat no:25747-1-AP)