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CDK6 Antibody

CDK6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19117-1-AP

Synonyms

CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, HeLa cells, C6 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 7 publications below

Product Information

The 19117-1-AP immunogen is Fusion Protein CDK6 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK6 fusion protein Ag5600
Full Name cyclin-dependent kinase 6
Calculated molecular weight 326aa,36 kDa
Observed molecular weight 36-40 kDa
GenBank accession number BC027989
Gene symbol CDK6
Gene ID (NCBI) 1021
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

Protocols

Product Specific Protocols
WB protocol for CDK6 antibody 19117-1-APDownload protocol
IHC protocol for CDK6 antibody 19117-1-APDownload protocol
IP protocol for CDK6 antibody 19117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biotechnol Appl Biochem

Knockdown of asparagine synthetase by RNAi suppresses cell growth in human melanoma cells and epidermoid carcinoma cells.

Authors - Hui Li
humanWB

Cancer Biother Radiopharm

γ-Glutamylcyclotransferase Knockdown Inhibits Growth of Lung Cancer Cells Through G0/G1 Phase Arrest.

Authors - Zhifeng Lin
humanWB

J Ophthalmol

LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04.

Authors - Yong Wang
humanWB

Onco Targets Ther

SUN1 silencing inhibits cell growth through G0/G1 phase arrest in lung adenocarcinoma.

Authors - Weiyi Huang
humanWB

Cancer Med

δ-Catenin regulates proliferation and apoptosis in renal cell carcinoma via promoting β-catenin nuclear localization and activating its downstream target genes.

Authors - Lincheng Ju
humanWB

Front Pharmacol

Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing β-Catenin Expression.

Authors - Yinshan Jin