Chromogranin A Antibody

Chromogranin A Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3H11C11

Cat no : 60135-1-Ig

Synonyms

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2



Tested Applications

Positive WB detected inrat pancreas tissue, pig pancreas tissue, human adrenal gland tissue, PC-3 cells
Positive IHC detected inhuman pancreas tissue, human colon tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:5000-1:80000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

60135-1-Ig targets Chromogranin A in IF, IHC, WB, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Reactivity human, rat, pig, mouse
Cited Species human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Chromogranin A fusion protein Ag0807
Full Name chromogranin A (parathyroid secretory protein 1)
Calculated molecular weight 51 kDa
Observed molecular weight 70 kDa
GenBank accession number BC006459
Gene symbol CHGA
Gene ID (NCBI) 1113
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Protocols

Product Specific Protocols
WB protocol for Chromogranin A antibody 60135-1-IgDownload protocol
IHC protocol for Chromogranin A antibody 60135-1-IgDownload protocol
IF protocol for Chromogranin A antibody 60135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Rep

The zinc transporter Zip14 (SLC39a14) affects Beta-cell Function: Proteomics, Gene expression, and Insulin secretion studies in INS-1E cells.

Authors - Trine Maxel
humanWB

Sci Rep

Cancer cell's neuroendocrine feature can be acquired through cell-cell fusion during cancer-neural stem cell interaction.

Authors - Liyuan Yin