CoraLite®555-conjugated Chromogranin A Monoclonal antibody

Chromogranin A Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3H11C11

Cat no : CL555-60135

Synonyms

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

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Validation Data Gallery

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  IF Staining of PC-12 using CL555-60135

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

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  IF Staining of SH-SY5Y using CL555-60135

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

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  IF Staining of Neuro-2a using CL555-60135

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL555-60135

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human Chromogranin A (CL555-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Neuro-2a using CL555-60135

  1X10^6 Neuro-2a cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human Chromogranin A (CL555-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: PC-12 cells, SH-SY5Y cells, Neuro-2a cells
• Positive FC detected in: SH-SY5Y cells, Neuro-2a cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-60135 targets Chromogranin A in IF, FC (Intra) applications and shows reactivity with human, rat, pig, mouse samples.

• Tested Reactivity: human, rat, pig, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Chromogranin A fusion protein Ag0807
• Full Name: chromogranin A (parathyroid secretory protein 1)
• Calculated Molecular Weight: 51 kDa
• GenBank Accession Number: BC006459
• Gene Symbol: CHGA
• Gene ID (NCBI): 1113
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.