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CHKA-Specific Polyclonal antibody

CHKA-Specific Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19537-1-AP

Synonyms

CHETK alpha, CHK, CHKA, CHKA-Specific, Choline kinase alpha, CK, CKI, EK, Ethanolamine kinase;

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of HepG2 using 19537-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of mouse testis using 19537-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of Raji using 19537-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of mouse testis using 19537-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of mouse testis using 19537-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of mouse colon using 19537-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of COLO 320 using 19537-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of COLO 320 using 19537-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of human testis using 19537-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    WB analysis of human testis using 19537-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    IHC staining of human breast cancer using 19537-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    IHC staining of human breast cancer using 19537-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    IHC staining of human liver using 19537-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CHKA-Specific Polyclonal antibody (19537-1-AP)

    IHC staining of human liver using 19537-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHKA-Specific Polyclonal antibody (19537-1-AP)

    IF Staining of HepG2 using 19537-1-AP

    Immunofluorescent analysis of HepG2 cells, using CHKA antibody 19537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, human testis tissue, mouse colon tissue, mouse testis tissue, Raji cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19537-1-AP targets CHKA-Specific in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name choline kinase alpha
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberNM_001277
Gene Symbol CHKA
Gene ID (NCBI) 1119
RRIDAB_10792247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.The antibody is specific to CHKA.

Protocols

Product Specific Protocols
WB protocol for CHKA-Specific antibody 19537-1-APDownload protocol
IHC protocol for CHKA-Specific antibody 19537-1-APDownload protocol
IF protocol for CHKA-Specific antibody 19537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols