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  • KD/KO Validated

CHRNA3 Antibody

CHRNA3 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 10333-1-AP

Synonyms

CHRNA3, LNCR2, NACHRA3, PAOD2



Tested Applications

Positive WB detected inmouse thymus tissue, A549 cells, HEK-293 cells, HepG2 cells, mouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10333-1-AP targets CHRNA3 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNA3 fusion protein Ag0272
Full Name cholinergic receptor, nicotinic, alpha 3
Calculated molecular weight 57 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000513
Gene symbol CHRNA3
Gene ID (NCBI) 1136
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHRNA3, also named as NACHRA3, LNCR2 and PAOD2, is neuronal type nAChR subunits. It is a member of the nicotinic acetylcholine receptor family, which plays an important role in calcium regulation, neuronal development, and cognitive functions. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer.

Protocols

Product Specific Protocols
WB protocol for CHRNA3 antibody 10333-1-APDownload protocol
IHC protocol for CHRNA3 antibody 10333-1-APDownload protocol
FC protocol for CHRNA3 antibody 10333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Nicotine induces fibrogenic changes in human liver via nicotinic acetylcholine receptors expressed on hepatic stellate cells.

Authors - Soeda Junpei J
humanWB

PLoS One

Nicotine Activates YAP1 through nAChRs Mediated Signaling in Esophageal Squamous Cell Cancer (ESCC).

Authors - Yue Zhao
  • KD Validated
humanFC

Mar Drugs

Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.

Authors - Zhihua Sun
mouseIHC

FASEB J

The gut-brain axis rewired: adding a functional vagal nicotinic "sensory synapse"

Authors - Azucena Perez-Burgos
humanWB

Hum Mol Genet

Transcriptome analysis and molecular signature of human retinal pigment epithelium.

Authors - Strunnikova N V NV