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CIDEB Polyclonal antibody

CIDEB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27600-1-AP

Synonyms

Cell death activator CIDE B, CIDEB

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CIDEB Polyclonal antibody (27600-1-AP)

    WB analysis using 27600-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27600-1-AP (CIDEB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CIDEB Polyclonal antibody (27600-1-AP)

    IHC staining of mouse liver using 27600-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27600-1-AP (CIDEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CIDEB Polyclonal antibody (27600-1-AP)

    IHC staining of mouse liver using 27600-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27600-1-AP (CIDEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

27600-1-AP targets CIDEB in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIDEB fusion protein Ag27003
Full Name cell death-inducing DFFA-like effector b
Calculated Molecular Weight 219 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC035970
Gene Symbol CIDEB
Gene ID (NCBI) 27141
RRIDAB_2880922
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell death‐inducing DFFA‐like effector B (CIDEB), an ER and lipid droplet (LD)‐associated protein primarily expressed in the liver, participated in lipid metabolism by regulating lipid droplet fusion and VLDL lipidation. Although CIDEA is expressed at high levels in brown adipose tissue (BAT) and CIDEC is highly enriched in white adipose tissue (WAT) and BAT, CIDEB is more abundant in liver, kidney, small intestine, and colon. CIDEB is localized to endoplasmic reticulum and lipid droplets. (PMID: 30858281, PMID: 24831470, PMID: 19187774)

Protocols

Product Specific Protocols
WB protocol for CIDEB antibody 27600-1-APDownload protocol
IHC protocol for CIDEB antibody 27600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Hazard Mater

TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis.

Authors - Changying Li
View Article
humanWB

Cancer Biol Ther

The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer

Authors - Wen-Jing Yun
View Article