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CILP2 Polyclonal antibody

CILP2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 11813-1-AP

Synonyms

CILP 2, CILP2, CLIP 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CILP2 Polyclonal antibody (11813-1-AP)

    WB analysis using 11813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CILP2 Polyclonal antibody (11813-1-AP)

    IF Staining of HEK-293 using 11813-1-AP

    Immunofluorescent analysis of HEK-293 cells using 11813-1-AP (CILP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, SW480 cells, mouse brain tissue
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11813-1-AP targets CILP2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CILP2 fusion protein Ag2366
Full Name cartilage intermediate layer protein 2
Calculated Molecular Weight 126 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC034926
Gene Symbol CILP2
Gene ID (NCBI) 148113
RRIDAB_2877795
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CILP2, also named as Cartilage intermediate layer protein 2, is a 1156 amino acid protein, which is expressed in articular chondrocytes but not in knee meniscal cartilage cells. CILP2 localizes to the intermediate to deep zone of articular cartilage. CILP2 may play a role in cartilage scaffolding. Given the full-length molecular mass of CILP2 is 125 kDa, but the most CILP2 protein in articular cartilage is cleaved,mostly likely at the furin cleavage site. CILP2 protein is detected as a major band on SDS-page migrating at about 90 kDa. (PMID: 21880736)

Protocols

Product Specific Protocols
WB protocol for CILP2 antibody 11813-1-APDownload protocol
IF protocol for CILP2 antibody 11813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development

Authors - Mengtian Zhang
View Article