Claudin 1 Antibody

Claudin 1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, dog and More (2)

Applications

IF, WB, ELISA

Conjugate

Unconjugated

Publications

36

Cat no : 13050-1-AP

Synonyms

claudin 1, CLD1, CLDN1, ILVASC, SEMP1



Tested Applications

Positive WB detected inA431 cells, mouse skin tissue, mouse thymus tissue, A549 cells, mouse liver tissue, HUVEC cells
Positive IF detected inHUVEC cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13050-1-AP targets Claudin 1 in IF, WB, ELISA applications and shows reactivity with human, mouse, dog samples.

Reactivity human, mouse, dog
Cited Species canine, dog, human, mouse, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 1 fusion protein Ag3713
Full Name claudin 1
Calculated molecular weight 211aa,23 kDa
Observed molecular weight 20-23 kDa
GenBank accession number BC012471
Gene symbol CLDN1
Gene ID (NCBI) 9076
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.

Protocols

Product Specific Protocols
WB protocol for Claudin 1 antibody 13050-1-APDownload protocol
IF protocol for Claudin 1 antibody 13050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Biol Macromol

Modulating the gut microbiota and inflammation is involved in the effect of Bupleurum polysaccharides against diabetic nephropathy in mice.

Authors - Yuchen Feng
humanWB

Biochim Biophys Acta Gene Regul Mech

The chromatin remodeling protein BRM regulates the transcription of tight junction proteins: Implication in breast cancer metastasis.

Authors - Yuyu Yang
mouseWB

Biochem Biophys Res Commun

Bacterial β-glucuronidase alleviates dextran sulfate sodium-induced colitis in mice: A possible crucial new diagnostic and therapeutic target for inflammatory bowel disease.

Authors - Yan He
swineWB

Microorganisms

Oral Administration of a Select Mixture of Lactobacillus and Bacillus Alleviates Inflammation and Maintains Mucosal Barrier Integrity in the Ileum of Pigs Challenged with Salmonella Infantis.

Authors - Xiao Liu
swineWB

Biomolecules

Succinate Modulates Intestinal Barrier Function and Inflammation Response in Pigs.

Authors - Xuan Li
mouseWB

J Cell Mol Med

Irisin reverses intestinal epithelial barrier dysfunction during intestinal injury via binding to the integrin αVβ5 receptor.

Authors - Jianbin Bi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua Morgan (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Keratinocytes
Claudin 1 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:13050-1-AP)