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CLEC4D Polyclonal antibody

CLEC4D Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 18930-1-AP

Synonyms

C type lectin like receptor 6, CD368, CLEC 6, CLEC4D, CLEC6, CLECSF8, MCL, MPCL

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood leukocyte using CLEC4D Polyclonal antibody (18930-1-AP)

    WB analysis of human peripheral blood leukocyte using 18930-1-AP

    Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 18930-1-AP (CLEC4D antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CLEC4D Polyclonal antibody (18930-1-AP)

    IHC staining of human tonsillitis using 18930-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18930-1-AP (CLEC4D Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CLEC4D Polyclonal antibody (18930-1-AP)

    IHC staining of human tonsillitis using 18930-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18930-1-AP (CLEC4D Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHuman peripheral blood leukocyte cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18930-1-AP is CLEC4D Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC4D fusion protein Ag6852
Full Name C-type lectin domain family 4, member D
Calculated Molecular Weight 215 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC032313
Gene Symbol CLEC4D
Gene ID (NCBI) 338339
RRIDAB_2878568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLEC4D antibody 18930-1-APDownload protocol
IHC protocol for CLEC4D antibody 18930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols