|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells|
|Positive IHC detected in||human prostate cancer tissue, human pancreas tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66110-1-Ig is COXIV Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||COXIV fusion protein Ag20551|
|Full Name||cytochrome c oxidase subunit IV isoform 1|
|Calculated molecular weight||19.6 kDa|
|Observed molecular weight||17 kDa|
|GenBank accession number||BC021236|
|Gene ID (NCBI)||1327|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.
|Product Specific Protocols|
|WB protocol for COXIV antibody 66110-1-Ig||Download protocol|
|IHC protocol for COXIV antibody 66110-1-Ig||Download protocol|
|IF protocol for COXIV antibody 66110-1-Ig||Download protocol|
|FC protocol for COXIV antibody 66110-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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