COXIV Monoclonal antibody

COXIV Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7B2

Cat no : 66110-1-Ig

Synonyms

Cell and organelle markers, COX IV 1, COX4, COX4I1, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker


"COXIV Antibodies" Comparison

View side-by-side comparison of COXIV antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • WB analysis using 66110-1-Ig

    WB analysis using 66110-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66110-1-Ig

    WB analysis using 66110-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66110-1-Ig

    WB analysis of HeLa using 66110-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66110-1-Ig

    IHC staining of human prostate cancer using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 66110-1-Ig

    IHC staining of human prostate cancer using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 66110-1-Ig

    IHC staining of human heart using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human prostate cancer using 66110-1-Ig

    IHC staining of human prostate cancer using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human prostate cancer using 66110-1-Ig

    IHC staining of human prostate cancer using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human pancreas using 66110-1-Ig

    IHC staining of human pancreas using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas using 66110-1-Ig

    IHC staining of human pancreas using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human pancreas using 66110-1-Ig

    IHC staining of human pancreas using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 66110-1-Ig

    IHC staining of human pancreas using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human heart using 66110-1-Ig

    IHC staining of human heart using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human heart using 66110-1-Ig

    IHC staining of human heart using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human heart using 66110-1-Ig

    IHC staining of human heart using 66110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens).

  • IF Staining of HepG2 using 66110-1-Ig

    IF Staining of HepG2 using 66110-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66110-1-Ig (COXIV antibody, 1:100) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L); Red is the staining with alpha Tubulin antibody (11224-1-AP, 1:100).

  • FC experiment of HeLa using 66110-1-Ig

    FC experiment of HeLa using 66110-1-Ig

    1X10^6 HeLa cells were stained with 0.2ug COXIV antibody (66110-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.


Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66110-1-Ig is COXIV Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COXIV fusion protein Ag20551
Full Name cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight19.6 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC021236
Gene symbol COX4I1
Gene ID (NCBI) 1327
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.

Protocols

Product Specific Protocols
WB protocol for COXIV antibody 66110-1-IgDownload protocol
IHC protocol for COXIV antibody 66110-1-IgDownload protocol
IF protocol for COXIV antibody 66110-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang
humanWB

Nat Cell Biol

Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS.

Authors - Yao Wang
humanWB

Mol Cell

Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery

Authors - Jasmin Adriana Schafer
mouseIF

Nat Commun

PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.

Authors - Ling Guo
ratWB

Microbiome

The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.

Authors - Weiping Xiao
humanWB

Elife

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

Authors - Maxence Le Vasseur

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bryce (Verified Customer) (02-18-2022)

Decent loading control for mitochondria. I cut the blot too low, but you get the point.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Kidney
COXIV Antibody Western Blot validation (1:1000 dilution) in Mouse Kidney (Cat no:66110-1-Ig)