COXIV Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11242-1-AP
COX IV 1, COX4, COX4I1, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial
Validation Data Gallery
|Positive WB detected in||HepG2 cells, A375 cells, A549 cells, HEK-293 cells, HeLa cells, human brain tissue, human lung tissue, human skeletal muscle tissue, human skin tissue, human spleen tissue, L02 cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, rat brain tissue, rat liver tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human prostate cancer tissue, human pancreas cancer tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11242-1-AP targets COXIV in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COXIV fusion protein Ag1640|
|Full Name||cytochrome c oxidase subunit IV isoform 1|
|Calculated molecular weight||19.6 kDa|
|Observed molecular weight||17-18 kDa|
|GenBank accession number||BC021236|
|Gene ID (NCBI)||1327|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.
|Product Specific Protocols|
|WB protocol for COXIV antibody 11242-1-AP||Download protocol|
|IHC protocol for COXIV antibody 11242-1-AP||Download protocol|
|IF protocol for COXIV antibody 11242-1-AP||Download protocol|
|IP protocol for COXIV antibody 11242-1-AP||Download protocol|
|FC protocol for COXIV antibody 11242-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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