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COXIV Polyclonal antibody

COXIV Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ChIP

Conjugate

Unconjugated

Cat no : 11242-1-AP

Synonyms

COX IV 1, COX4, COX4I1, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial


"COXIV Antibodies" Comparison

View side-by-side comparison of COXIV antibodies from other vendors to find the one that best suits your research needs.
  • IF Staining of HepG2 using 11242-1-AP

    IF Staining of HepG2 using 11242-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11242-1-AP (COXIV antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 11242-1-AP

    IF Staining of HepG2 using 11242-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV rabbit pAb (11242-1-AP) at dilution of 1:100 and alpha Tubulin mouse mAb (66031-1-Ig) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 11242-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66031-1-Ig.

  • WB analysis using 11242-1-AP

    WB analysis using 11242-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11242-1-AP

    IHC staining of human prostate cancer using 11242-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 11242-1-AP

    IHC staining of human prostate cancer using 11242-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 11242-1-AP

    FC experiment of HepG2 using 11242-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human COXIV (11242-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

  • IP experiment of mouse skeletal muscle using 11242-1-AP

    IP experiment of mouse skeletal muscle using 11242-1-AP

    IP Result of anti-COXIV (IP:11242-1-AP, 3ug; Detection:11242-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3500ug.


Tested Applications

Positive WB detected inHeLa cells, A375 cells, A549 cells, HEK-293 cells, HepG2 cells, human brain tissue, human lung tissue, human skeletal muscle tissue, human skin tissue, human spleen tissue, L02 cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, rat brain tissue, rat liver tissue, Jurkat cells, NIH/3T3 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman prostate cancer tissue, human pancreas cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11242-1-AP targets COXIV in WB, IP, IHC, IF, FC, ChIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity goat, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COXIV fusion protein Ag1640
Full Name cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight19.6 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC021236
Gene symbol COX4I1
Gene ID (NCBI) 1327
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.

Protocols

Product Specific Protocols
WB protocol for COXIV antibody 11242-1-APDownload protocol
IHC protocol for COXIV antibody 11242-1-APDownload protocol
IF protocol for COXIV antibody 11242-1-APDownload protocol
IP protocol for COXIV antibody 11242-1-APDownload protocol
FC protocol for COXIV antibody 11242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Nutr Food Res

PMID: 30632304

Authors - Wenguang Chang
humanIF

Bioconjug Chem

Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.

Authors - Yipeng Du
humanWB

Nat Commun

The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.

Authors - Biao Ma
ratWB

Int J Radiat Oncol Biol Phys

Phenylephrine Alleviates 131I Radiation Damage in Submandibular Gland through Maintaining Mitochondrial Homeostasis.

Authors - Xin Yue Wang
mouseWB

Haematologica

Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis.

Authors - Zengdi Zhang
mouseWB

Autophagy

Deficiency of mitophagy receptor FUNDC1 impairs mitochondrial quality and aggravates dietary-induced obesity and metabolic syndrome.

Authors - Hao Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (08-17-2021)

Good ab, works well 1:1000 in BSA 3% O/N 4ºC incubation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human primary fibroblasts
COXIV Antibody Western Blot validation (1:1000 dilution) in human primary fibroblasts (Cat no:11242-1-AP)
FH

Mohammed (Verified Customer) (08-19-2020)

Very strong staining in the midpiece of the sperm tail.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: (1:200)
  • Cell Tissue Type: Isolated sperm
FH

SITING (Verified Customer) (07-13-2020)

THIS IS A GOOD ANTIBODY, CAN SEE CLEAR BAND

  • Applications: Western Blot,
COXIV Antibody Western Blot, validation ( dilution) in (Cat no:11242-1-AP)