COXIV Monoclonal antibody, PBS Only

COXIV Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7B2

Cat no : 66110-1-PBS

Synonyms

Cell and organelle markers, COX IV 1, COX4, COX4I1, COXIV, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker

---

Validation Data Gallery

• 

  WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66110-1-Ig (same clone as 66110-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66110-1-Ig (COXIV antibody, 1:100) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L); Red is the staining with alpha Tubulin antibody (11224-1-AP, 1:100). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66110-1-Ig (same clone as 66110-1-PBS)

  1X10^6 HeLa cells were stained with 0.2ug COXIV antibody (66110-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66110-1-PBS targets COXIV in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: COXIV fusion protein Ag20551
• Full Name: cytochrome c oxidase subunit IV isoform 1
• Calculated Molecular Weight: 19.6 kDa
• Observed Molecular Weight: 17-18 kDa
• GenBank Accession Number: BC021236
• Gene Symbol: COX4I1
• Gene ID (NCBI): 1327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.