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CPLX3 Polyclonal antibody

CPLX3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16949-1-AP

Synonyms

complexin 3, Complexin III, CPLX3, CPX III, CPXIII, Nbla11589

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPLX3 Polyclonal antibody (16949-1-AP)

    WB analysis using 16949-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16949-1-AP (CPLX3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CPLX3 Polyclonal antibody (16949-1-AP)

    WB analysis of human brain using 16949-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16949-1-AP (CPLX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CPLX3 Polyclonal antibody (16949-1-AP)

    IP experiment of mouse brain using 16949-1-AP

    IP result of anti-CPLX3 (IP:16949-1-AP, 3ug; Detection:16949-1-AP 1:300) with mouse brain tissue lysate 6000ug.

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16949-1-AP targets CPLX3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPLX3 fusion protein Ag10571
Full Name complexin 3
Calculated Molecular Weight 158 aa, 18 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC018026
Gene Symbol CPLX3
Gene ID (NCBI) 594855
RRIDAB_2084042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPLX3, also named as Complexin-3, is 158 amino acid protein, which belongs to the complexin/synaphin family. CPLX3 localizes in the membrane and binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A. CPLX3 positively regulates a late step in synaptic vesicle exocytosis.

Protocols

Product Specific Protocols
WB protocol for CPLX3 antibody 16949-1-APDownload protocol
IP protocol for CPLX3 antibody 16949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Proc Natl Acad Sci U S A

Extracortical origin of some murine subplate cell populations.

Authors - María Pedraza
View Article
mouseWB

Free Radic Biol Med

NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.

Authors - Wang Ziying Z
View Article