|Positive WB detected in||mouse brain tissue, human liver tissue, rat brain tissue, L02 cells, HeLa cells|
|Positive IHC detected in||human gliomas tissue, human colon tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11448-1-AP targets COX5A in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chimpanzee, human, monkey, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COX5A fusion protein Ag2005|
|Full Name||cytochrome c oxidase subunit Va|
|Calculated molecular weight||150 aa, 17 kDa|
|Observed molecular weight||13 kDa|
|GenBank accession number||BC024240|
|Gene ID (NCBI)||9377|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COX5A(Cytochrome c oxidase subunit 5A,mitochondrial)is also named as COXVA and belongs to the cytochrome c oxidase subunit 5A family.This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).
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