COX5A Polyclonal antibody

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• COX5A Polyclonal antibody (11448-1-AP)

COX5A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11448-1-AP Copied!

Publications (26)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

COX VA, COX5, COXVA

• 

  WB analysis of mouse brain using 11448-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 11448-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at 4 degree celsius over night.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11448-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 11448-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11448-1-AP (COX5A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11448-1-AP (COX5A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11448-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX5A antibody (11448-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, rat brain tissue, SH-SY5Y cells
• Positive IHC detected in: human colon tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11448-1-AP targets COX5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, zebrafish, chimpanzee
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2005

  Product name: Recombinant human COX5A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-150 aa of BC024240

  Sequence: MLGAALRRCAVAATTRADPRGLLHSARTPGPAVAIQSVRCYSHGSQETDEEFDARWVTYFNKPDIDAWELRKGINTLVTYDMVPEPKIIDAALRACRRLNDFASTVRILEVVKDKAGPHKEIYPYVIQELRPTLNELGISTPEELGLDKV

• Full Name: cytochrome c oxidase subunit Va
• Calculated Molecular Weight: 150 aa, 17 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC024240
• Gene Symbol: COX5A
• Gene ID (NCBI): 9377
• RRID: AB_2085429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20674
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX5A(Cytochrome c oxidase subunit 5A, mitochondrial)is also named COXVA and belongs to the cytochrome c oxidase subunit 5A family. This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).

Publications

What published studies show

Anti-COX5A (Catalog No. 11448-1-AP) has 26 total citations. Publications appear in journals including Cancer Research, Nucleic Acids Research, Journal of Biological Chemistry, Cell Death & Disease, Developmental Cell, Advanced Science, Human Molecular Genetics, and International Journal of Molecular Sciences. Associated research fields encompass mitochondrial biology, cancer, cardiovascular disease, neurodegeneration, ophthalmology, and metabolic disorders.

Automatically generated from the 26 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Res; CK1δ/CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma; Authors - Karen L Burger
zebrafish		Nucleic Acids Res; Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.; Authors - Danni Chen
	WB	Nucleic Acids Res; Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.; Authors - Qinghai Zhang
	WB	J Biomed Sci; Optimized allotopic expression of mitochondrial ND6 transgene restored complex I and apoptosis deficiencies caused by LHON-linked ND6 14484T > C mutation; Authors - Jing Wang
	WB	Adv Sci (Weinh); Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption; Authors - Fan Ouyang
rat	WB	Cell Death Dis; Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.; Authors - Yuehong Wang