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CYP1A1 Polyclonal antibody

CYP1A1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13241-1-AP

Synonyms

AHH, AHRR, CP11, CYP1, CYP1A1, CYPIA1, Cytochrome P450 1A1, Cytochrome P450 C, Cytochrome P450 form 6, Cytochrome P450 P1, P1 450, P450 C, P450DX



Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse lung tissue, HepG2 cells, HeLa cells, K-562 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13241-1-AP targets CYP1A1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse, rat, zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP1A1 fusion protein Ag3990
Full Name cytochrome P450, family 1, subfamily A, polypeptide 1
Calculated molecular weight 512 aa, 58 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberBC023019
Gene symbol CYP1A1
Gene ID (NCBI) 1543
RRIDAB_2877928
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CYP1A1 antibody 13241-1-APDownload protocol
IHC protocol for CYP1A1 antibody 13241-1-APDownload protocol
IF protocol for CYP1A1 antibody 13241-1-APDownload protocol
IP protocol for CYP1A1 antibody 13241-1-APDownload protocol
FC protocol for CYP1A1 antibody 13241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Environ Int

Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR)

Authors - Séverine A Degrelle
human,mouseWB

Free Radic Biol Med

5-Methoxyflavone ameliorates non-alcoholic fatty liver disease through targeting the cytochrome P450 1A1

Authors - Yurou Zhang
human,mouseWB

Phytother Res

Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity

Authors - Xiaojing Wang
humanWB

Environ Pollut

Regioselective hydroxylation of carbendazim by mammalian cytochrome P450: A combined experimental and computational study.

Authors - Xia Lv
humanWB,IHC

BMC Med

NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma

Authors - Yizhi Zhan
humanWB,IF

J Gastroenterol

Ferroptosis in the colon epithelial cells as a therapeutic target for ulcerative colitis

Authors - Akihito Yokote