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CYP1A1 Polyclonal antibody

CYP1A1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13241-1-AP

Synonyms

AHH, AHRR, CP11, CYP1, CYP1A1, CYPIA1, Cytochrome P450 1A1, Cytochrome P450 C, Cytochrome P450 form 6, Cytochrome P450 P1, P1 450, P450 C, P450DX



Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse lung tissue, HepG2 cells, HeLa cells, K-562 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13241-1-AP targets CYP1A1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP1A1 fusion protein Ag3990
Full Name cytochrome P450, family 1, subfamily A, polypeptide 1
Calculated molecular weight 512 aa, 58 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberBC023019
Gene symbol CYP1A1
Gene ID (NCBI) 1543
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP1A1 antibody 13241-1-APDownload protocol
IHC protocol for CYP1A1 antibody 13241-1-APDownload protocol
IF protocol for CYP1A1 antibody 13241-1-APDownload protocol
IP protocol for CYP1A1 antibody 13241-1-APDownload protocol
FC protocol for CYP1A1 antibody 13241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Pharm Sci

PMID: 30776468

Authors - Vinay R Sonawane

J Biomed Res

Hydroxylation and sulfation of sex steroid hormones in inflammatory liver.

Authors - Sang R Lee
humanWB

ACS Omega

Khellinoflavanone, a Semisynthetic Derivative of Khellin, Overcomes Benzo[a]pyrene Toxicity in Human Normal and Cancer Cells That Express CYP1A1.

Authors - Rajni Sharma
humanWB

Int J Mol Epidemiol Genet

Chrysophanol protects human bronchial epithelial cells from cigarette smoke extract (CSE)-induced apoptosis.

Authors - Guorao Wu
humanWB

Signal Transduct Target Ther

AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma.

Authors - Qing Zhu
humanWB

Xenobiotica

A high-throughput cell-based gaussia luciferase reporter assay for measurement of CYP1A1, CYP2B6, and CYP3A4 induction.

Authors - Han Li