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- KD/KO Validated
CYP1A1 Polyclonal antibody
CYP1A1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 13241-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse cerebellum tissue, mouse lung tissue, HepG2 cells, HeLa cells, K-562 cells |
Positive IP detected in | mouse lung tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 40 publications below |
IHC | See 6 publications below |
IF | See 3 publications below |
Product Information
13241-1-AP targets CYP1A1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Reactivity | human, mouse, pig |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CYP1A1 fusion protein Ag3990 |
Full Name | cytochrome P450, family 1, subfamily A, polypeptide 1 |
Calculated molecular weight | 512 aa, 58 kDa |
Observed molecular weight | 50-58 kDa |
GenBank accession number | BC023019 |
Gene symbol | CYP1A1 |
Gene ID (NCBI) | 1543 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
IHC protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
IF protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
IP protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
FC protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Free Radic Biol Med 5-Methoxyflavone ameliorates non-alcoholic fatty liver disease through targeting the cytochrome P450 1A1 | ||
Environ Int Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR) | ||
Environ Pollut Regioselective hydroxylation of carbendazim by mammalian cytochrome P450: A combined experimental and computational study. | ||
BMC Med NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma | ||
Int J Mol Sci Intestinal Stem Cells Damaged by Deoxycholic Acid via AHR Pathway Contributes to Mucosal Barrier Dysfunction in High-Fat Feeding Mice | ||
Free Radic Biol Med Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells. |