CYP1A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYP1A1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

47

Cat no : 13241-1-AP

Synonyms

AHH, AHRR, CP11, CYP1, CYP1A1, CYPIA1, Cytochrome P450 1A1, Cytochrome P450 C, Cytochrome P450 form 6, Cytochrome P450 P1, P1 450, P450 C, P450DX

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 13241-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13241-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13241-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13241-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13241-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 13241-1-AP

  IP Result of anti-CYP1A1 (IP:13241-1-AP, 4ug; Detection:13241-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

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  IHC staining of human breast cancer using 13241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13241-1-AP (CYP1A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 13241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13241-1-AP (CYP1A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13241-1-AP

  Immunofluorescent analysis of HeLa cells using 13241-1-AP (CYP1A1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 13241-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug CYP1A1 antibody (13241-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, mouse lung tissue, HepG2 cells, HeLa cells, K-562 cells
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 40 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Product Information

13241-1-AP targets CYP1A1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP1A1 fusion protein Ag3990
• Full Name: cytochrome P450, family 1, subfamily A, polypeptide 1
• Calculated molecular weight: 512 aa, 58 kDa
• Observed molecular weight: 50-58 kDa
• GenBank accession number: BC023019
• Gene symbol: CYP1A1
• Gene ID (NCBI): 1543
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Free Radic Biol Med; 5-Methoxyflavone ameliorates non-alcoholic fatty liver disease through targeting the cytochrome P450 1A1; Authors - Yurou Zhang
human	WB,IF	Environ Int; Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR); Authors - Séverine A Degrelle
human	WB	Environ Pollut; Regioselective hydroxylation of carbendazim by mammalian cytochrome P450: A combined experimental and computational study.; Authors - Xia Lv
human	WB,IHC	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan
mouse	WB	Int J Mol Sci; Intestinal Stem Cells Damaged by Deoxycholic Acid via AHR Pathway Contributes to Mucosal Barrier Dysfunction in High-Fat Feeding Mice; Authors - Leheng Liu
human	WB	Free Radic Biol Med; Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.; Authors - Restituto Tocmo