CYP1A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYP1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

86

Cat no : 13241-1-AP

Synonyms

Cytochrome P450 1A1, CYPIA1, CYP1, CP11, AHRR

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 13241-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13241-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13241-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13241-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13241-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13241-1-AP

  IP result of anti-CYP1A1 (IP:13241-1-AP, 4ug; Detection:13241-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

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  IHC staining of human breast cancer using 13241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13241-1-AP (CYP1A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 13241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13241-1-AP (CYP1A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13241-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP1A1 antibody (13241-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 13241-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CYP1A1 Polyclonal antibody (13241-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, mouse cerebellum tissue, mouse lung tissue, rat liver tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 72 publications below
• IHC: See 10 publications below
• IF: See 12 publications below

Product Information

13241-1-AP targets CYP1A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP1A1 fusion protein Ag3990
• Full Name: cytochrome P450, family 1, subfamily A, polypeptide 1
• Calculated Molecular Weight: 512 aa, 58 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: BC023019
• Gene Symbol: CYP1A1
• Gene ID (NCBI): 1543
• RRID: AB_2877928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Environ Int; Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR); Authors - Séverine A Degrelle
human,mouse	WB	Free Radic Biol Med; 5-Methoxyflavone ameliorates non-alcoholic fatty liver disease through targeting the cytochrome P450 1A1; Authors - Yurou Zhang
human,mouse	WB	Phytother Res; Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity; Authors - Xiaojing Wang
mouse	IF	NPJ Regen Med; Myeloid cell-associated aromatic amino acid metabolism facilitates CNS myelin regeneration; Authors - Jingwen Hu
human	WB	Environ Pollut; Regioselective hydroxylation of carbendazim by mammalian cytochrome P450: A combined experimental and computational study.; Authors - Xia Lv
human	WB,IHC	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan