SCAMP2 Polyclonal antibody

SCAMP2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15119-1-AP

Synonyms

SCAMP2

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Validation Data Gallery

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  WB analysis of HepG2 using 15119-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15119-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15119-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15119-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15119-1-AP

  IP result of anti-SCAMP2 (IP:15119-1-AP, 4ug; Detection:15119-1-AP 1:500) with HepG2 cells lysate 3300 ug.

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  IHC staining of human pancreas cancer using 15119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15119-1-AP (SCAMP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 15119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15119-1-AP (SCAMP2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 15119-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SCAMP2 antibody (15119-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 15119-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug SCAMP2 antibody (15119-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, human liver tissue, mouse lung tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15119-1-AP targets SCAMP2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCAMP2 fusion protein Ag7190
• Full Name: secretory carrier membrane protein 2
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC001376
• Gene Symbol: SCAMP2
• Gene ID (NCBI): 10066
• RRID: AB_2184772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCAMP2 functions in post-Golgi recycling pathways. It acts as a recycling carrier to the cell surface. It couples Arf6-stimulated PLD activity to exocytosis and links this process to formation of fusion pores. SCAMP2 is marker for secretory vesicles and tobacco BY-2 cells as a model system. The MW of SCAMP2 is 36-39 kDa with modification.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.; Authors - Feiyu Mao