SCAMP2 Polyclonal antibody

SCAMP2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15119-1-AP

Synonyms

SCAMP2



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human liver tissue, mouse lung tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15119-1-AP targets SCAMP2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCAMP2 fusion protein Ag7190
Full Name secretory carrier membrane protein 2
Calculated molecular weight 37 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC001376
Gene symbol SCAMP2
Gene ID (NCBI) 10066
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCAMP2 functions in post-Golgi recycling pathways. It acts as a recycling carrier to the cell surface. It couples Arf6-stimulated PLD activity to exocytosis and links this process to formation of fusion pores. SCAMP2 is marker for secretory vesicles and tobacco BY-2 cells as a model system. The MW of SCAMP2 is 36-39 kDa with modification.

Protocols

Product Specific Protocols
WB protocol for SCAMP2 antibody 15119-1-APDownload protocol
IHC protocol for SCAMP2 antibody 15119-1-APDownload protocol
IF protocol for SCAMP2 antibody 15119-1-APDownload protocol
IP protocol for SCAMP2 antibody 15119-1-APDownload protocol
FC protocol for SCAMP2 antibody 15119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.

Authors - Feiyu Mao