CRHR1 Polyclonal antibody

CRHR1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20967-1-AP

Synonyms

CRF R, CRF R 1, CRF R1, CRF1, CRF1R, CRFR, CRFR 1, CRFR1, CRH R 1, CRH R1, CRH R1h, CRHR, CRHR1, CRHR1f



Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20967-1-AP targets CRHR1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name corticotropin releasing hormone receptor 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 46-55 kDa
GenBank Accession NumberNM_004382
Gene Symbol CRHR1
Gene ID (NCBI) 1394
RRIDAB_2878776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Corticotropin-releasing hormone (CRH, also known as Corticotropin-releasing factor, CRF) is a 41 amino acid peptide hormone and a key regulator of the stress response. CRH and the related urocortin peptides (UCN, UCN2 and UCN3) mediate their actions through two mammalian receptor subtypes, CRHR1 and CRHR2 (PMID: 23863939). CRHR1 (also known as CRFR1) exhibits high affinity towards CRH and UCN but low affinity towards UCN2 and no affinity towards UCN3 (PMID: 22684850). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Widely distributed throughout the brain, CRHR1 critically controls behavioral adaptation to stress and is causally linked to emotional disorders (PMID: 21885734). Alternative splicing results in multiple transcript variants encoding distinct isoforms of CRHR1. This antibody raised against a synthesized peptide corresponding to amino acid residues 148-165 of isoform CRF-R1 can only recognize isoform CRF-R1.

Protocols

Product Specific Protocols
WB protocol for CRHR1 antibody 20967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Proc Natl Acad Sci U S A

Overactivation of corticotropin-releasing factor receptor type 1 and aquaporin-4 by hypoxia induces cerebral edema.

Authors - Shao-Jun Chen
humanWB,IF

Pharmaceuticals (Basel)

A Novel Mast Cell Stabilizer JM25-1 Rehabilitates Impaired Gut Barrier by Targeting the Corticotropin-Releasing Hormone Receptors

Authors - Yueshan Sun
WB

Behav Brain Res

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Authors - Shuai Lian
WB

Brain Res

Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Authors - Di Wang
WB

Int J Mol Med

Sini San ameliorates duodenal mucosal barrier injury and low‑grade inflammation via the CRF pathway in a rat model of functional dyspepsia.

Authors - Chunyang Zhu
WB

Reprod Toxicol

Dynamic expression of corticotropin-releasing hormone and urocortin in estrogen induced-cholestasis pregnant rat.

Authors - Fan Zhou