CRYBB3 Antibody

CRYBB3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21009-1-AP

Synonyms

Beta B3 crystallin, Beta crystallin B3, CATCN2, CRYB3, CRYBB3, crystallin, beta B3



Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The 21009-1-AP immunogen is Fusion Protein CRYBB3 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRYBB3 fusion protein Ag15212
Full Name crystallin, beta B3
Calculated molecular weight 211aa,24 kDa
Observed molecular weight 24 kDa
GenBank accession number BC069479
Gene symbol CRYBB3
Gene ID (NCBI) 1417
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CRYBB3 antibody 21009-1-APDownload protocol
IHC protocol for CRYBB3 antibody 21009-1-APDownload protocol
IF protocol for CRYBB3 antibody 21009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Lab Invest

Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway.

Authors - Wenting Yu