Alpha E-Catenin Polyclonal antibody

Alpha E-Catenin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12831-1-AP

Synonyms

alpha catenin, Alpha E catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, α-catenin, α-E-catenin, αE-catenin



Tested Applications

Positive WB detected inmouse uterus tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue, human colon tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12831-1-AP targets Alpha E-Catenin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha E-Catenin fusion protein Ag3565
Full Name catenin (cadherin-associated protein), alpha 1, 102kDa
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC031262
Gene symbol CTNNA1
Gene ID (NCBI) 1495
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Protocols

Product Specific Protocols
WB protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
IHC protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
IF protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
IP protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
FC protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Helicobacter

Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.

Authors - Chen Zhang
humanWB

Exp Ther Med

GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial-mesenchymal transition and metastasis through regulation of SLUG.

Authors - Yibin Zhou
humanWB

Med Oncol

Wnt signaling regulation of stem-like properties in human lung adenocarcinoma cell lines.

Authors - Yan Zhang
human

Oncol Rep

Downregulation of Bmi-1 suppresses epithelial‑mesenchymal transition in melanoma.

Authors - Yanting Liu
humanWB

Cancer Sci

NUBPL, a novel metastasis-related gene, promotes colorectal carcinoma cell motility by inducing epithelial-mesenchymal transition.

Authors - Yuhui Wang
humanIF

Sci Rep

SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth.

Authors - Rehab Alshargabi