CUL4A Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66038-1-Ig |CloneNo.: 1A7F12

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Freight/Packing

Con: 114 μg/150 μl

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Species specificity:
human, monkey, mouse, rat, pig

Positive WB detected in:
pig brain tissue,HeLa cells

Positive IP detected in:
MCF-7 cells

Positive IHC detected in:
human heart tissue, human breast cancer tissue, human heart tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
cullin 4A

Calculated molecular weight:
77 kDa

Observed molecular weight:
77 kDa; 88 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CUL4A

Synonyms
CUL 4A, CUL4A, cullin 4A
Background

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.


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