CUL4A-Specific Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 14851-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 45 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
HeLa cells, HL-60 cells, Jurkat cells, mouse thymus tissue

Positive IHC detected in:
human liver cancer tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
cullin 4A

Calculated molecular weight:
88 kDa

Observed molecular weight:
88 kDa,77 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CUL4A

Synonyms
CUL 4A, CUL4A, cullin 4A
Background

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. This antibody is specially against CUL4A,but not CUL4B. CUL4A has 2 isoforms with MW 88 kDa (759 aa) and 77kDa (659 aa), and Catalog #14851-1-AP recognizes both.


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