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  • KD/KO Validated

CUL4A-Specific Antibody

CUL4A-Specific Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14851-1-AP

Synonyms

CUL 4A, CUL4A, cullin 4A



Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, Jurkat cells, mouse thymus tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14851-1-AP targets CUL4A-Specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cullin 4A
Calculated molecular weight 88 kDa
Observed molecular weight 88 kDa,77 kDa
GenBank accession number NM_003589
Gene symbol CUL4A
Gene ID (NCBI) 8451
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. This antibody is specially against CUL4A,but not CUL4B. CUL4A has 2 isoforms with MW 88 kDa (759 aa) and 77kDa (659 aa), and Catalog #14851-1-AP recognizes both.

Protocols

Product Specific Protocols
WB protocol for CUL4A-Specific antibody 14851-1-APDownload protocol
IHC protocol for CUL4A-Specific antibody 14851-1-APDownload protocol
IF protocol for CUL4A-Specific antibody 14851-1-APDownload protocol
FC protocol for CUL4A-Specific antibody 14851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3.

Authors - Xiaoyan Hu
  • KD Validated
humanWB

PLoS Genet

Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.

Authors - Haitao Sun
mouseWB

J Biol Chem

APP may act as a substrate and a recognition unit for CRL4CRBN and Stub1 E3 ligases facilitating ubiquitination of proteins involved in presynaptic functions and neurodegeneration.

Authors - Dolores Del Prete
ratWB

Oxid Med Cell Longev

Cul4a as a New Interaction Protein of PARP1 Inhibits Oxidative Stress-Induced H9c2 Cell Apoptosis.

Authors - Ning Ye
  • KD Validated
humanWB

J Med Chem

Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction.

Authors - Wenjuan Zhou
mouseWB

Nat Commun

CSN6 drives carcinogenesis by positively regulating Myc stability.

Authors - Jian Chen