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CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20882-1-AP

Synonyms



Tested Applications

Positive WB detected inmouse thymus tissue, HeLa cells, SH-SY5Y cells, Jurkat cells, Y79 cells, human testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman tonsillitis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20882-1-AP is CUL4B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL4B fusion protein Ag15024
Full Name cullin 4B
Calculated molecular weight 913 aa, 104 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC036216
Gene symbol CUL4B
Gene ID (NCBI) 8450
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B.

Protocols

Product Specific Protocols
WB protocol for CUL4B antibody 20882-1-APDownload protocol
IHC protocol for CUL4B antibody 20882-1-APDownload protocol
IF protocol for CUL4B antibody 20882-1-APDownload protocol
IP protocol for CUL4B antibody 20882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols