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  • Western Blot (WB) analysis of various lysates using CUL4B Polyclonal antibody (20882-1-AP)

    WB analysis using 20882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CUL4B Polyclonal antibody (20882-1-AP)

    WB analysis of HeLa cells using 20882-1-AP

    WB result of CUL4B antibody (20882-1-AP, 1:500) with si-Control and si-CUL4B transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CUL4B Polyclonal antibody (20882-1-AP)

    WB analysis using 20882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CUL4B Polyclonal antibody (20882-1-AP)

    WB analysis of SH-SY5Y using 20882-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using CUL4B Polyclonal antibody (20882-1-AP)

    WB analysis of Y79 using 20882-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CUL4B Polyclonal antibody (20882-1-AP)

    IP experiment of mouse brain using 20882-1-AP

    IP result of anti-CUL4B (IP:20882-1-AP, 4ug; Detection:20882-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CUL4B Polyclonal antibody (20882-1-AP)

    IHC staining of human tonsillitis using 20882-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CUL4B Polyclonal antibody (20882-1-AP)

    IHC staining of human tonsillitis using 20882-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CUL4B Polyclonal antibody (20882-1-AP)

    IHC staining of human gliomas using 20882-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CUL4B Polyclonal antibody (20882-1-AP)

    IHC staining of human gliomas using 20882-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CUL4B Polyclonal antibody (20882-1-AP)

    IHC staining of human gliomas using 20882-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CUL4B Polyclonal antibody (20882-1-AP)

    IHC staining of human gliomas using 20882-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUL4B Polyclonal antibody (20882-1-AP)

    IF Staining of HeLa using 20882-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (20882-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CUL4B Polyclonal antibody (20882-1-AP)

    IF Staining of HepG2 using 20882-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CUL4B antibody (20882-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUL4B Polyclonal antibody (20882-1-AP)

    IF Staining of HeLa using 20882-1-AP

    Immunofluorescent analysis of Hela cells, using CUL4B antibody 20882-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CUL4B Polyclonal antibody (20882-1-AP)

    IF Staining of HepG2 using 20882-1-AP

    Immunofluorescent analysis of HepG2 cells, using CUL4B antibody 20882-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Featured Product
  • KD/KO Validated

CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20882-1-AP
Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KIAA0695, Cullin-4B, cullin 4B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Tested Applications

Positive WB detected inmouse thymus tissue, Jurkat cells, Y79 cells, SH-SY5Y cells, HeLa cells, rat thymus tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman tonsillitis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20882-1-AP targets CUL4B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15024

Product name: Recombinant human CUL4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-195 aa of BC036216

Sequence: MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAK

Predict reactive species
Full Name cullin 4B
Calculated Molecular Weight 913 aa, 104 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
RRIDAB_10694831
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13620
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B.

Protocols

Product Specific Protocols
IF protocol for CUL4B antibody 20882-1-APDownload protocol
IHC protocol for CUL4B antibody 20882-1-APDownload protocol
IP protocol for CUL4B antibody 20882-1-APDownload protocol
WB protocol for CUL4B antibody 20882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Neuropsychopharmacology

Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.

Authors - Gonca Bayraktar
  • KD Validated
View Article
mouseIP

Front Immunol

PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.

Authors - Nianyin Lv
View Article
mouseIF

Cells

Cullin4 E3 Ubiquitin Ligases Regulate Male Gonocyte Migration, Proliferation and Blood-Testis Barrier Homeostasis.

Authors - Yan Yin
View Article
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