Collagen Type IV Polyclonal antibody

Collagen Type IV Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55131-1-AP

Synonyms

COL4A2, Collagen alpha 2(IV) chain, Collagen Type IV, collagen, type IV, alpha 2



Tested Applications

Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

55131-1-AP targets Collagen Type IV in IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name collagen, type IV, alpha 2
Calculated molecular weight 168 kDa
GenBank accession numberNM_001846
Gene symbol COL4A2
Gene ID (NCBI) 1284
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Collagen Type IV antibody 55131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

J Cell Mol Med

Chemerin/ChemR23 axis promotes inflammation of glomerular endothelial cells in diabetic nephropathy.

Authors - Jin Shang

Biosci Rep

5-aminoisoquinoline improves renal function and fibrosis during recovery phase of cisplatin-induced acute kidney injury in rats.

Authors - Andrés Quesada
humanWB

EBioMedicine

Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.

Authors - Tianjiao Wang
ratWB

Mol Ther Nucleic Acids

lncRNA NR_038323 Suppresses Renal Fibrosis in Diabetic Nephropathy by Targeting the miR-324-3p/DUSP1 Axis.

Authors - Yanni Ge
mouseWB

Diabetes Metab Syndr Obes

Ursolic Acid Treatment Alleviates Diabetic Kidney Injury By Regulating The ARAP1/AT1R Signaling Pathway.

Authors - Tian-Kui Ma
ratWB

Oxid Med Cell Longev

Human Tissue Kallikrein 1 Improves Erectile Dysfunction of Streptozotocin-Induced Diabetic Rats by Inhibition of Excessive Oxidative Stress and Activation of the PI3K/AKT/eNOS Pathway.

Authors - Yang Luan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua (Verified Customer) (04-28-2019)

Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HMEC1
Collagen Type IV Antibody Immunofluorescence, validation (1:500 dilution) in HMEC1 (Cat no:55131-1-AP)