|Positive IHC detected in||human kidney tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
55131-1-AP targets Collagen Type IV in IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, swine|
|Host / Isotype||Rabbit / IgG|
|Full Name||collagen, type IV, alpha 2|
|Calculated molecular weight||168 kDa|
|GenBank accession number||NM_001846|
|Gene ID (NCBI)||1284|
|Purification Method||Antigen affinity purification|
|Storage Buffer||0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joshua (Verified Customer) (04-28-2019)
Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.