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WB analysis using 55131-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55131-1-AP (COL4A2-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 55131-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 55131-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast hyperplasia using 55131-1-AP
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 55131-1-AP (COL4A2-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast hyperplasia using 55131-1-AP
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 55131-1-AP (COL4A2-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 55131-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 55131-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 55131-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 55131-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human kidney using 55131-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using COL4A2-specific antibody (55131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human kidney using 55131-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using COL4A2-specific antibody (55131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

COL4A2 Polyclonal antibody

Name: COL4A2 Polyclonal antibody
Brand: Proteintech
SKU: 55131-1-AP
Rating: 4.80 (5 reviews)

COL4A2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 55131-1-AP

Publications(71)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

COL4A2-specific, Canstatin, Collagen Ⅳ, Collagen alpha-2(IV) chain, Collagen Type IV

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (71)
Protocols
Reviews (5)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IHC detected in	human kidney tissue, human breast hyperplasia tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 22 publications below
IF	See 14 publications below

Product Information

55131-1-AP targets COL4A2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	collagen, type IV, alpha 2
Calculated Molecular Weight	168 kDa
Observed Molecular Weight	200 kDa
GenBank Accession Number	NM_001846
Gene Symbol	COL4A2
Gene ID (NCBI)	1284
RRID	AB_2881273
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08572
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COL4A2 antibody 55131-1-AP	Download protocol
IHC protocol for COL4A2 antibody 55131-1-AP	Download protocol
IF protocol for COL4A2 antibody 55131-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		EMBO J Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress. Authors - Simon Schwörer View Article
human		EBioMedicine Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma. Authors - Tianjiao Wang View Article
	IF	ACS Appl Mater Interfaces Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function. Authors - Yi Li View Article
human	IF	Biomaterials Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy. Authors - Menghao Shi View Article
human	IHC	Biofabrication Engineering microvasculature by 3D bioprinting of prevascularized spheroids in photo-crosslinkable gelatin. Authors - Lise De Moor View Article
		Cell Mol Life Sci Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion Authors - Shiju Ye View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Samantha (Verified Customer) (05-10-2024)	Clean bands detected in DRG and Spinal cord tissue of mice. Applications: Western Blot Primary Antibody Dilution: 1 in 1000 and 1 in 5000 Cell Tissue Type: Dorsal Root Ganglion, Spinal Cord
FH Pankhuri (Verified Customer) (08-22-2022)	Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully. Applications: Immunofluorescence Cell Tissue Type: NSC organoid COL4A2 Antibody Immunofluorescence validation ( dilution) in NSC organoid (Cat no:55131-1-AP)
FH Lindsey (Verified Customer) (05-24-2021)	BULLETPROOF ANTIBODY! Applications: Immunohistochemistry, Immunofluorescence Primary Antibody Dilution: 1:500 Cell Tissue Type: Human kidney (FFPE) COL4A2 Antibody Immunohistochemistry,Immunofluorescence validation (1:500 dilution) in Human kidney (FFPE) (Cat no:55131-1-AP)
FH Malak (Verified Customer) (02-24-2021)	This antibody works very well Applications: Western Blot
FH Joshua (Verified Customer) (04-28-2019)	Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes. Applications: Immunofluorescence, Primary Antibody Dilution: 1:500 Cell Tissue Type: HMEC1 COL4A2 Antibody Immunofluorescence, validation (1:500 dilution) in HMEC1 (Cat no:55131-1-AP)