Collagen Type IV Polyclonal antibody
Collagen Type IV Polyclonal Antibody for IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 55131-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 27 publications below |
| IHC | See 9 publications below |
| IF | See 3 publications below |
Product Information
55131-1-AP targets Collagen Type IV in IHC, IF,ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | collagen, type IV, alpha 2 |
| Calculated molecular weight | 168 kDa |
| GenBank accession number | NM_001846 |
| Gene symbol | COL4A2 |
| Gene ID (NCBI) | 1284 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Collagen Type IV antibody 55131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Chemerin/ChemR23 axis promotes inflammation of glomerular endothelial cells in diabetic nephropathy. | ||
Biosci Rep 5-aminoisoquinoline improves renal function and fibrosis during recovery phase of cisplatin-induced acute kidney injury in rats. | ||
EBioMedicine Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma. | ||
Mol Ther Nucleic Acids lncRNA NR_038323 Suppresses Renal Fibrosis in Diabetic Nephropathy by Targeting the miR-324-3p/DUSP1 Axis. | ||
Diabetes Metab Syndr Obes Ursolic Acid Treatment Alleviates Diabetic Kidney Injury By Regulating The ARAP1/AT1R Signaling Pathway. | ||
Oxid Med Cell Longev Human Tissue Kallikrein 1 Improves Erectile Dysfunction of Streptozotocin-Induced Diabetic Rats by Inhibition of Excessive Oxidative Stress and Activation of the PI3K/AKT/eNOS Pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Malak (Verified Customer) (02-24-2021) | This antibody works very well
|
FH Joshua (Verified Customer) (04-28-2019) | Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.
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