Connexin-26 Polyclonal antibody

Connexin-26 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16960-1-AP

Synonyms

Connexin 26, Connexin-26, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, Gap junction beta 2 protein, GJB2, HID, KID, NSRD1, PPK



Tested Applications

Positive WB detected inPC-3 cells, human liver tissue
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16960-1-AP targets Connexin-26 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Connexin-26 fusion protein Ag10666
Full Name gap junction protein, beta 2, 26kDa
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC017048
Gene symbol GJB2
Gene ID (NCBI) 2706
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin-26 (GJB2) is a beta class gap junction protein. Mutations in the gene for connexin-26 are responsible for as much as 50% of pre-lingual, recessive deafness.

Protocols

Product Specific Protocols
WB protocol for Connexin-26 antibody 16960-1-APDownload protocol
IF protocol for Connexin-26 antibody 16960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Med Rep

Molecular hydrogen accelerates the reversal of acute obstructive cholangitis‑induced liver dysfunction by restoring gap and tight junctions.

Authors - Zhiyang Zhu