- Featured Product
- KD/KO Validated
DRP1 (C-terminal) Polyclonal antibody
DRP1 (C-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 12957-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, HepG2 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 34 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
12957-1-AP targets DRP1 (C-terminal) in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | chicken, hamster, human, monkey, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DRP1 (C-terminal) fusion protein Ag3644 |
| Full Name | dynamin 1-like |
| Calculated molecular weight | 710 aa, 79 kDa |
| Observed molecular weight | 75-80 kDa |
| GenBank accession number | BC024590 |
| Gene symbol | DNM1L |
| Gene ID (NCBI) | 10059 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
| IHC protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
| IF protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
| IP protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Cyclooxygenase-2-Mediated Up-Regulation of Mitochondrial Transcription Factor A Mitigates the Radio-Sensitivity of Cancer Cells. | ||
Ecotoxicol Environ Saf Destruction of redox and mitochondrial dynamics co-contributes to programmed cell death in chicken kidney under arsenite or/and copper (II) exposure. | ||
Oncotarget Porcine reproductive and respiratory syndrome virus triggers mitochondrial fission and mitophagy to attenuate apoptosis.
| ||
Oncotarget Antagonistic effects of selenium on cadmium-induced apoptosis by restoring the mitochondrial dynamic equilibrium and energy metabolism in chicken spleens. | ||
Oxid Med Cell Longev Exercise Combined with Rhodiola sacra Supplementation Improves Exercise Capacity and Ameliorates Exhaustive Exercise-Induced Muscle Damage through Enhancement of Mitochondrial Quality Control. | ||
Aging (Albany NY) CHIP protects against MPP+/MPTP-induced damage by regulating Drp1 in two models of Parkinson's disease. |
