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KD/KO Validated

DRP1 (C-terminal) Polyclonal antibody

Name: DRP1 (C-terminal) Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 12957-1-AP

DRP1 (C-terminal) Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12957-1-AP

Synonyms

DLP1, DNM1L, DRP1

Validation Data Gallery

WB analysis using 12957-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12957-1-AP
WB result of DRP1 (C-terminal) antibody (12957-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HeLa cells.
IHC staining of mouse cerebellum using 12957-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 12957-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12957-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 12957-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human DRP1 (C-terminal) (12957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.
IP experiment of mouse brain using 12957-1-AP
IP result of anti-DRP1 (C-terminal)(IP:12957-1-AP, 4ug; Detection:12957-1-AP 1:1000) with mouse brain tissue lysate 2400 ug.

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Cat No. 12957-1-AP

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Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, HepG2 cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 38 publications below
IHC	See 4 publications below
IF	See 2 publications below

Product Information

12957-1-AP targets DRP1 (C-terminal) in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	chicken, hamster, human, monkey, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DRP1 (C-terminal) fusion protein Ag3644
Full Name	dynamin 1-like
Calculated molecular weight	710 aa, 79 kDa
Observed molecular weight	75-80 kDa
GenBank accession number	BC024590
Gene symbol	DNM1L
Gene ID (NCBI)	10059
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DRP1 (C-terminal) antibody 12957-1-AP	Download protocol
IHC protocol for DRP1 (C-terminal) antibody 12957-1-AP	Download protocol
IF protocol for DRP1 (C-terminal) antibody 12957-1-AP	Download protocol
IP protocol for DRP1 (C-terminal) antibody 12957-1-AP	Download protocol
FC protocol for DRP1 (C-terminal) antibody 12957-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Int J Mol Sci Cyclooxygenase-2-Mediated Up-Regulation of Mitochondrial Transcription Factor A Mitigates the Radio-Sensitivity of Cancer Cells. Authors - Fan Tang View Article
chicken	WB	Ecotoxicol Environ Saf Destruction of redox and mitochondrial dynamics co-contributes to programmed cell death in chicken kidney under arsenite or/and copper (II) exposure. Authors - Hongjing Zhao View Article
monkey	WB	Oncotarget Porcine reproductive and respiratory syndrome virus triggers mitochondrial fission and mitophagy to attenuate apoptosis. Authors - Shuaifeng Li KD Validated View Article
human	WB	Oncotarget Antagonistic effects of selenium on cadmium-induced apoptosis by restoring the mitochondrial dynamic equilibrium and energy metabolism in chicken spleens. Authors - Zhe Xu View Article
mouse	WB	Oxid Med Cell Longev Exercise Combined with Rhodiola sacra Supplementation Improves Exercise Capacity and Ameliorates Exhaustive Exercise-Induced Muscle Damage through Enhancement of Mitochondrial Quality Control. Authors - Yaoshan Dun View Article
mouse	WB	Aging (Albany NY) CHIP protects against MPP+/MPTP-induced damage by regulating Drp1 in two models of Parkinson's disease. Authors - Zhengwei Hu View Article