Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 12957-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12957-1-AP
WB result of DRP1 (C-terminal) antibody (12957-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HeLa cells.
WB analysis using 12957-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12957-1-AP
IP result of anti-DRP1 (C-terminal) (IP:12957-1-AP, 4ug; Detection:12957-1-AP 1:1000) with mouse brain tissue lysate 2400 ug.
IHC staining of mouse cerebellum using 12957-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 12957-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12957-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DRP1 (C-terminal) antibody (12957-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 12957-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DRP1 (C-terminal) (12957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

DRP1 (C-terminal) Polyclonal antibody

DRP1 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12957-1-AP

Publications(305)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

DRP1, DLP1, DNM1L, DNM1L,DLP1, Dnm1p/Vps1p-like protein

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (305)
Protocols
Reviews (4)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, A549 cells, rat brain tissue, DU 145 cells, HCT 116 cells, HepG2 cells, NIH/3T3 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 286 publications below
IHC	See 22 publications below
IF	See 31 publications below
IP	See 4 publications below
CoIP	See 2 publications below

Product Information

12957-1-AP targets DRP1 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, chicken, zebrafish, bovine, hamster, goat, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DRP1 (C-terminal) fusion protein Ag3644 Predict reactive species
Full Name	dynamin 1-like
Calculated Molecular Weight	736 aa, 82 kDa
Observed Molecular Weight	75-80 kDa
GenBank Accession Number	BC024590
Gene Symbol	DRP1
Gene ID (NCBI)	10059
RRID	AB_2093525
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00429
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DRP1 (C-terminal) antibody 12957-1-AP	Download protocol
IHC protocol for DRP1 (C-terminal) antibody 12957-1-AP	Download protocol
IF protocol for DRP1 (C-terminal) antibody 12957-1-AP	Download protocol
IP protocol for DRP1 (C-terminal) antibody 12957-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression Authors - Shiqiao Qiu View Article
human	WB,IP,IHC	Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy Authors - Yang Meng View Article
mouse	WB	Cell Stem Cell Mechano-oncogenic cytoskeletal remodeling drives leukemic transformation with mitochondrial vesicle-mediated STING activation Authors - Zemin Song KO Validated View Article
human	WB	Cell Metab Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae. Authors - Jing Yuan View Article
mouse	WB	Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice Authors - He-Zhou Huang View Article
mouse	WB,IF	Bioact Mater Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis Authors - Wei Zhang KD Validated View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH P (Verified Customer) (09-23-2024)	Very Good Applications: Western Blot Primary Antibody Dilution: 1000 Cell Tissue Type: HEK DRP1 (C-terminal) Antibody Western Blot validation (1000 dilution) in HEK (Cat no:12957-1-AP)
FH Mi (Verified Customer) (03-06-2023)	It works pretty well in human white adipocyte cells. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: adipocytes
FH Samrajni (Verified Customer) (04-27-2022)	The product works well. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: C2C12 cell line
FH Tom (Verified Customer) (07-07-2021)	Paraformaldehyde fixation and Triton X-100 permeabilization. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: HeLa DRP1 (C-terminal) Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:12957-1-AP)