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DRP1 (C-terminal) Antibody 15 Publications

Rabbit Polyclonal| Catalog number: 12957-1-AP

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Con: 24 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

DRP1 (C-terminal) Antibody WB mouse brain tissue 12957-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

DRP1 (C-terminal) Antibody WB HeLa cells 12957-1-AP

WB result of DRP1 (C-terminal) antibody (12957-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HeLa cells

DRP1 (C-terminal) Antibody IHC mouse cerebellum tissue 12957-1-AP

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

DRP1 (C-terminal) Antibody IHC mouse cerebellum tissue 12957-1-AP

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

DRP1 (C-terminal) Antibody IF HeLa cells 12957-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

DRP1 (C-terminal) Antibody IP mouse brain tissue 12957-1-AP

IP result of anti-DRP1 (C-terminal)(IP:12957-1-AP, 4ug; Detection:12957-1-AP 1:1000) with mouse brain tissue lysate 2400 ug

Species specificity:
human, mouse, chicken, monkey
Positive WB detected in:
mouse brain tissue, NIH/3T3 cells, HepG2 cells, Transfected HEK-293 cells
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
mouse cerebellum tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
DRP1 (C-terminal) fusion protein Ag3644
Full name:
dynamin 1-like
Calculated molecular weight:
710aa,79 kDa
Observed molecular weight:
75-80 kDa
GenBank accession number:
BC024590
Gene ID (NCBI):
10059
Gene symbol
DNM1L
Synonyms
DLP1, DNM1L, DRP1

Background

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.


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