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DRP1 (C-terminal) Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 12957-1-AP

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Con: 24 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

DRP1 (C-terminal) Antibody WB mouse brain tissue 12957-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

DRP1 (C-terminal) Antibody IP mouse brain tissue 12957-1-AP

IP result of anti-DRP1 (C-terminal)(IP:12957-1-AP, 4ug; Detection:12957-1-AP 1:1000) with mouse brain tissue lysate 2400 ug

DRP1 (C-terminal) Antibody IF HeLa cells 12957-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, chicken, monkey
Positive WB detected in:
mouse brain tissue, HepG2 cells, NIH/3T3 cells, Transfected HEK-293 cells
Positive IP detected in:
mouse brain tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
DRP1 (C-terminal) fusion protein Ag3644
Full name:
dynamin 1-like
Calculated molecular weight:
710aa,79 kDa
Observed molecular weight:
75-80 kDa
GenBank accession number:
BC024590
Gene ID (NCBI):
10059
Gene symbol
DNM1L
Synonyms
DLP1, DNM1L, DRP1

Background

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.


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