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DNM2 Polyclonal antibody

DNM2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14605-1-AP

Synonyms

CMTDI1, CMTDIB, DI CMTB, DNM2, DYN2, dynamin 2, DYNII

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNM2 Polyclonal antibody (14605-1-AP)

    WB analysis using 14605-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DNM2 Polyclonal antibody (14605-1-AP)

    WB analysis of NIH/3T3 using 14605-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DNM2 Polyclonal antibody (14605-1-AP)

    WB analysis of HeLa using 14605-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DNM2 Polyclonal antibody (14605-1-AP)

    WB analysis of Jurkat using 14605-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DNM2 Polyclonal antibody (14605-1-AP)

    WB analysis of human brain using 14605-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, human brain tissue, MDA-MB-231 cells, human peripheral blood platelets, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14605-1-AP targets DNM2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNM2 fusion protein Ag6156
Full Name dynamin 2
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC054501
Gene Symbol DNM2
Gene ID (NCBI) 1785
RRIDAB_2277414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.

Protocols

Product Specific Protocols
WB protocol for DNM2 antibody 14605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Pharmacol

CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.

Authors - Fang Bian
View Article
humanWB

J Transl Med

Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure.

Authors - Jing Liu
View Article
mouseIF

Aging Cell

Degradation of cochlear Connexin26 accelerate the development of age-related hearing loss

Authors - Kai Xu
View Article