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E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

6B11F11

Cat no : 60335-1-Ig

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin



Tested Applications

Positive WB detected inPC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60335-1-Ig targets E-cadherin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, monkey, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag15085
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated molecular weight 882 aa, 97 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC141838
Gene symbol CDH1
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protocols

Product Specific Protocols
WB protocol for E-cadherin antibody 60335-1-IgDownload protocol
IHC protocol for E-cadherin antibody 60335-1-IgDownload protocol
IF protocol for E-cadherin antibody 60335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

BMC Cancer

miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα.

Authors - He Xin
humanWB,IF

Cancer Cell Int

SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition.

Authors - Zhou Yang
humanIF

Hum Reprod

Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.

Authors - Yanling Gou
humanWB

Am J Transl Res

VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.

Authors - Adheesh Bhandari
humanWB,IF

J Exp Clin Cancer Res

RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer.

Authors - Qingyuan Li
humanWB,IHC

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua (Verified Customer) (12-27-2019)

Caco-2 cells fixed in 4% paraformaldehyde. Stained overnight at 4C. Bright stain, minimal background

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Caco-2
E-cadherin Antibody Immunofluorescence, validation (1:500 dilution) in Caco-2 (Cat no:60335-1-Ig)
FH

Louisiane (Verified Customer) (02-06-2019)

Cells were fixed with 4% PFA for 10 min, permeabilized with 0.1% Triton-X100 for 5 min and blocked with 1% FBS/1% BSA in PBS for 3 h. Antibodies were diluted in 1% FBS/1% BSA in PBS. Primary antibody: 2 h. Alexa Fluor anti-mouse secondary antibody (1:250): 1 h. Cells were imaged by confocal microscopy - no labeling was observed.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: MCF7