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E-cadherin Antibody

E-cadherin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

6B11F11

Cat no : 60335-1-Ig

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin



Tested Applications

Positive WB detected inPC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60335-1-Ig targets E-cadherin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, monkey, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag15085
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated molecular weight 882aa,97 kDa
Observed molecular weight 120 kDa
GenBank accession number BC141838
Gene symbol CDH1
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protocols

Product Specific Protocols
WB protocol for E-cadherin antibody 60335-1-IgDownload protocol
IHC protocol for E-cadherin antibody 60335-1-IgDownload protocol
IF protocol for E-cadherin antibody 60335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

BMC Cancer

miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα.

Authors - He Xin
humanWB,IF

Cancer Cell Int

SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition.

Authors - Zhou Yang
humanIF

Hum Reprod

Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.

Authors - Yanling Gou
humanWB

Am J Transl Res

VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.

Authors - Adheesh Bhandari
humanWB,IF

J Exp Clin Cancer Res

RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer.

Authors - Qingyuan Li
humanWB,IHC

EBioMedicine

Cinobufotalin powerfully reversed EBV-miR-BART22-induced cisplatin resistance via stimulating MAP2K4 to antagonize non-muscle myosin heavy chain IIA/glycogen synthase 3β/β-catenin signaling pathway.

Authors - Yiyi Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua (Verified Customer) (12-27-2019)

Caco-2 cells fixed in 4% paraformaldehyde. Stained overnight at 4C. Bright stain, minimal background

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Caco-2
E-cadherin Antibody Immunofluorescence, validation (1:500 dilution) in Caco-2 (Cat no:60335-1-Ig)
FH

Louisiane (Verified Customer) (02-06-2019)

Cells were fixed with 4% PFA for 10 min, permeabilized with 0.1% Triton-X100 for 5 min and blocked with 1% FBS/1% BSA in PBS for 3 h. Antibodies were diluted in 1% FBS/1% BSA in PBS. Primary antibody: 2 h. Alexa Fluor anti-mouse secondary antibody (1:250): 1 h. Cells were imaged by confocal microscopy - no labeling was observed.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: MCF7
No image submitted with review