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ECHS1 Antibody

ECHS1 Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B9D5

Cat no : 66117-1-Ig

Synonyms

ECHS1, Enoyl CoA hydratase 1, SCEH



Tested Applications

Positive WB detected inHeLa cells, human testis tissue, PC-3 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The 66117-1-Ig immunogen is Fusion Protein ECHS1 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ECHS1 fusion protein Ag16775
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession number BC008906
Gene symbol ECHS1
Gene ID (NCBI) 1892
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

Protocols

Product Specific Protocols
WB protocol for ECHS1 antibody 66117-1-IgDownload protocol
IHC protocol for ECHS1 antibody 66117-1-IgDownload protocol
IP protocol for ECHS1 antibody 66117-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Ann Clin Transl Neurol

Deficiency of ECHS1 causes mitochondrial encephalopathy with cardiac involvement.

Authors - Tobias B Haack
humanWB

J Proteomics

Up-regulation of fatty acid oxidation in the ligament as a contributing factor of ankylosing spondylitis: A comparative proteomic study.

Authors - Wei-Dong Xu