Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, human testis tissue, MCF-7 cells, PC-3 cells, HEK-293 cells, L02 cells, COLO 320 cells, T47D cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66117-1-Ig targets ECHS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16775 Product name: Recombinant human ECHS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-290 aa of BC008906 Sequence: MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ Predict reactive species |
| Full Name | enoyl Coenzyme A hydratase, short chain, 1, mitochondrial |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC008906 |
| Gene Symbol | ECHS1 |
| Gene ID (NCBI) | 1892 |
| RRID | AB_2881516 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P30084 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ECHS1 antibody 66117-1-Ig | Download protocol |
| IHC protocol for ECHS1 antibody 66117-1-Ig | Download protocol |
| IP protocol for ECHS1 antibody 66117-1-Ig | Download protocol |
| WB protocol for ECHS1 antibody 66117-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-ECHS1 antibody (Cat# 66117-1-Ig) has 9 citations across journals including Cell Death Dis, Cell Reports, Brain Communications, Annals of Clinical and Translational Neurology, Journal of Proteomics, FEBS Open Bio, Journal of Cancer, Fetal and Pediatric Pathology, and bioRxiv. Research fields span colorectal cancer, KRAS-driven cancer, mitochondrial encephalopathy, fatty acid metabolism, ankylosing spondylitis, non-alcoholic fatty liver disease, epilepsy, and pediatric genetic diagnostics. Applications include WB, IF, IP, and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis ECHS1, an interacting protein of LASP1, induces sphingolipid-metabolism imbalance to promote colorectal cancer progression by regulating ceramide glycosylation.
| ||
Cell Rep Mutant KRAS-driven selective mRNA translation reveals mechanisms and therapeutic vulnerabilities in cancer. | ||
Brain Commun Neurological, metabolic and inflammatory phenotypes in a mouse model of ECHS1 deficiency. | ||
Ann Clin Transl Neurol Deficiency of ECHS1 causes mitochondrial encephalopathy with cardiac involvement. | ||
J Cancer Knockdown of HOXD13 in Oral Squamous Cell Carcinoma Inhibited its Proliferation, Migration, and Influenced Fatty Acid Metabolism | ||
J Proteomics Up-regulation of fatty acid oxidation in the ligament as a contributing factor of ankylosing spondylitis: A comparative proteomic study. |
