ECHS1 Monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

  WB result of ECHS1 antibody (66117-1-Ig, 1:20,000) with si-Control and si-ECHS1 transfected HepG2 cells. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  WB analysis using 66117-1-Ig (same clone as 66117-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66117-1-Ig (same clone as 66117-1-PBS)

  WB result of ECHS1 antibody (66117-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66117-1-Ig (same clone as 66117-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66117-1-Ig (same clone as 66117-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

  IP result of anti-ECHS1 (IP:66117-1-Ig, 3ug; Detection:66117-1-Ig 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ECHS1 antibody (66117-1-Ig, Clone: 2B9D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ECHS1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 66117-1-PBS

Clone No.2B9D5

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

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Product Information

66117-1-PBS targets ECHS1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16775

  Product name: Recombinant human ECHS1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-290 aa of BC008906

  Sequence: MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ

• Full Name: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC008906
• Gene Symbol: ECHS1
• Gene ID (NCBI): 1892
• RRID: AB_2881516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30084
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)