EXT2 Polyclonal antibody

EXT2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11348-1-AP

Synonyms

exostoses (multiple) 2, Exostosin 2, EXT2, Multiple exostoses protein 2, SOTV



Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells, U-937 cells, C6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11348-1-AP targets EXT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXT2 fusion protein Ag1748
Full Name exostoses (multiple) 2
Calculated Molecular Weight 718 aa, 82 kDa
Observed Molecular Weight 70-82 kDa
GenBank Accession NumberBC010058
Gene Symbol EXT2
Gene ID (NCBI) 2132
RRIDAB_2293839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Exostosin-2 (EXT2) is type II transmembrane glycosyltransferase forming with EXT1 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone(PMID: 22660413, 36402845, 36593275).

Protocols

Product Specific Protocols
WB protocol for EXT2 antibody 11348-1-APDownload protocol
IHC protocol for EXT2 antibody 11348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome

Authors - Valeria De Pasquale