|Positive WB detected in||A375 cells, human brain tissue, mouse skin tissue|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||U2OS cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12348-1-AP targets FABP5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FABP5 fusion protein Ag3005|
|Full Name||fatty acid binding protein 5 (psoriasis-associated)|
|Calculated molecular weight||135 aa, 15 kDa|
|Observed molecular weight||15 kDa|
|GenBank accession number||BC019385|
|Gene ID (NCBI)||2171|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)
|Product Specific Protocols|
|WB protocol for FABP5 antibody 12348-1-AP||Download protocol|
|IHC protocol for FABP5 antibody 12348-1-AP||Download protocol|
|IF protocol for FABP5 antibody 12348-1-AP||Download protocol|
|FC protocol for FABP5 antibody 12348-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Head Neck Oncol
Identification of potential therapeutic targets in human head & neck squamous cell carcinoma.
Deregulating the CYP2C19/Epoxy-Eicosatrienoic Acid-Associated FABP4/FABP5 Signaling Network as a Therapeutic Approach for Metastatic Triple-Negative Breast Cancer.
Eur J Pharmacol
FABP5 is correlated with poor prognosis and promotes tumour cell growth and metastasis in clear cell renal cell carcinoma.
Int J Mol Sci
Resveratrol Suppresses the Growth and Enhances Retinoic Acid Sensitivity of Anaplastic Thyroid Cancer Cells.
Mol Biol Cell
Fatty-acid Binding Protein 5 modulates the SAR1 GTPase cycle and enhances budding of large COPII cargos.
J Agric Food Chem
Lysine Enhances the Stimulation of Fatty Acids on Milk Fat Synthesis via the GPRC6A-PI3K-FABP5 Signaling in Bovine Mammary Epithelial Cells.