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  • Featured Product
  • KD/KO Validated

FARSA Polyclonal antibody

FARSA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18121-1-AP

Synonyms

CML33, FARS, FARSA, FARSL, FARSLA, FRSA, PheHA, PheRS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FARSA Polyclonal antibody (18121-1-AP)

    WB analysis using 18121-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FARSA Polyclonal antibody (18121-1-AP)

    WB analysis using 18121-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FARSA Polyclonal antibody (18121-1-AP)

    WB analysis of K-562 using 18121-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using FARSA Polyclonal antibody (18121-1-AP)

    WB analysis of MCF-7 using 18121-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using FARSA Polyclonal antibody (18121-1-AP)

    IP experiment of MCF-7 using 18121-1-AP

    IP result of anti-FARSA (IP:18121-1-AP, 4ug; Detection:18121-1-AP 1:2000) with MCF-7 cells lysate 3240 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FARSA Polyclonal antibody (18121-1-AP)

    IHC staining of human liver cancer using 18121-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FARSA Polyclonal antibody (18121-1-AP)

    IHC staining of human liver cancer using 18121-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FARSA Polyclonal antibody (18121-1-AP)

    IHC staining of human kidney using 18121-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FARSA Polyclonal antibody (18121-1-AP)

    IHC staining of human kidney using 18121-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FARSA Polyclonal antibody (18121-1-AP)

    IF Staining of MCF-7 using 18121-1-AP

    Immunofluorescent analysis of MCF-7 cells, using FARSA antibody 18121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FARSA Polyclonal antibody (18121-1-AP)

    IF Staining of HeLa using 18121-1-AP

    Immunofluorescent analysis of Hela cells, using FARSA antibody 18121-1-AP at 1:25 dilution and FITC- labeled Donkey anti-Rabbit IgG (green).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FARSA Polyclonal antibody (18121-1-AP)

    IF Staining of HeLa using 18121-1-AP

    Immunofluorescent analysis of Hela cells, using FARSA antibody 18121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, HepG2 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18121-1-AP targets FARSA in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FARSA fusion protein Ag12647
Full Name phenylalanyl-tRNA synthetase, alpha subunit
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC006495
Gene Symbol FARSA
Gene ID (NCBI) 2193
RRIDAB_2102509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FARSA antibody 18121-1-APDownload protocol
IHC protocol for FARSA antibody 18121-1-APDownload protocol
IF protocol for FARSA antibody 18121-1-APDownload protocol
IP protocol for FARSA antibody 18121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
View Article
humanWB

Clin Genet

FARS1-related disorders caused by bi-allelic mutations in cytosolic phenylalanyl-tRNA synthetase genes: Look beyond the lungs!

Authors - Luise A Schuch
View Article
human,rat,mouseIF,ELISA

Nat Commun

Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.

Authors - Qian Zhou
  • KO Validated
View Article
humanWB,IP

Autoimmun Rev

Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase

Authors - Yoshinao Muro
View Article
human,mouseWB,IF,RIP

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj
View Article