FARSA Antibody

FARSA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 18121-1-AP

Synonyms

CML33, FARS, FARSA, FARSL, FARSLA, FRSA, PheHA, PheRS



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, HepG2 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

18121-1-AP targets FARSA in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FARSA fusion protein Ag12647
Full Name phenylalanyl-tRNA synthetase, alpha subunit
Calculated molecular weight 58 kDa
Observed molecular weight 57 kDa
GenBank accession number BC006495
Gene symbol FARSA
Gene ID (NCBI) 2193
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FARSA antibody 18121-1-APDownload protocol
IHC protocol for FARSA antibody 18121-1-APDownload protocol
IF protocol for FARSA antibody 18121-1-APDownload protocol
IP protocol for FARSA antibody 18121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols