FGF1 Polyclonal antibody

FGF1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17400-1-AP

Synonyms

AFGF, ECGF, ECGF beta, ECGFA, ECGFB, FGF alpha, FGF1, FGFA, GLIO703, HBGF 1, HBGF1



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17400-1-AP targets FGF1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGF1 fusion protein Ag11366
Full Name fibroblast growth factor 1 (acidic)
Calculated molecular weight 155 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC032697
Gene symbol FGF1
Gene ID (NCBI) 2246
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FGF1 antibody 17400-1-APDownload protocol
IHC protocol for FGF1 antibody 17400-1-APDownload protocol
IF protocol for FGF1 antibody 17400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols