FGFR1 Monoclonal antibody

FGFR1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

10F12E7

Cat no : 60325-1-Ig

Synonyms

bFGF R 1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR1, FLG, FLT 2, FLT2, Fms like tyrosine kinase 2, HBGFR, KAL2, N SAM, OGD, Proto oncogene c Fgr



Tested Applications

Positive WB detected inMCF-7 cells, HT-1080 cells, HUVEC cells, U2OS cells, Y79 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inACHN cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60325-1-Ig targets FGFR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen FGFR1 fusion protein Ag3646
Full Name fibroblast growth factor receptor 1
Calculated Molecular Weight 820 aa, 91 kDa
Observed Molecular Weight 91 kDa
GenBank Accession NumberBC018128
Gene Symbol FGFR1
Gene ID (NCBI) 2260
RRIDAB_2881435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for FGFR1 antibody 60325-1-IgDownload protocol
IHC protocol for FGFR1 antibody 60325-1-IgDownload protocol
IF protocol for FGFR1 antibody 60325-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Authors - Chunqing Wang
IHC

ACS Appl Mater Interfaces

Hydrogel Loaded with Peptide-Containing Nanocomplexes: Symphonic Cooperation of Photothermal Antimicrobial Nanoparticles and Prohealing Peptides for the Treatment of Infected Wounds

Authors - Qiuye Jia
humanWB

Theranostics

Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.

Authors - Meng Wang
humanWB,IHC

J Transl Med

A novel intronic circular RNA circFGFR1int2 up-regulates FGFR1 by recruiting transcriptional activators P65/FUS and suppressing miR-4687-5p to promote prostate cancer progression

Authors - Ruyue Wang
humanWB

Oxid Med Cell Longev

DJ-1/FGFR-1 Signaling Pathway Contributes to Sorafenib Resistance in Hepatocellular Carcinoma.

Authors - Xin Chen
humanWB

Int J Mol Sci

The FGFR Family Inhibitor AZD4547 Exerts an Antitumor Effect in Ovarian Cancer Cells.

Authors - Yu Ran Na