Tested Applications
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13042-1-AP targets FGFR2/CD332 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3701 Product name: Recombinant human FGFR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 358-704 aa of BC039243 Sequence: EFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPPGMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDINNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTNEEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT Predict reactive species |
| Full Name | fibroblast growth factor receptor 2 |
| Calculated Molecular Weight | 709 aa, 79 kDa |
| Observed Molecular Weight | |
| GenBank Accession Number | BC039243 |
| Gene Symbol | FGFR2 |
| Gene ID (NCBI) | 2263 |
| RRID | AB_10642943 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21802 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. This polyclonal antibody raised against 358-704aa of human FGFR2 can cross-react with other members of the FGFR family.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FGFR2/CD332 antibody 13042-1-AP | Download protocol |
| IF protocol for FGFR2/CD332 antibody 13042-1-AP | Download protocol |
| IHC protocol for FGFR2/CD332 antibody 13042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
FGFR2/CD332 Polyclonal antibody (Cat# 13042-1-AP) has 12 citations published in journals including J Transl Med, Cells, Int J Mol Sci, Invest Ophthalmol Vis Sci, Life, Stem Cells Int, Am J Cancer Res, Tissue Eng Part A, Nephron, Bioengineered, Anim Biotechnol, and J Craniofac Surg. Research fields span hepatocellular carcinoma, brain radiation injury, breast cancer, dry eye disease, esophageal cancer, pulmonary fibrosis, oral squamous cell carcinoma, uterine repair, diabetic nephropathy, osteoblast differentiation, hair follicle biology, and cranial suture development.
| Species | Application | Title |
|---|---|---|
J Transl Med A novel murine model of combined hepatocellular carcinoma and intrahepatic cholangiocarcinoma | ||
Cells Distinct Metabolomic and Proteomic Signatures of Early Brain Damage Triggered by the Entrance Plateau Versus the Spread-Out Bragg Peak of Proton Radiation. | ||
Int J Mol Sci Using Cancer-Associated Fibroblasts as a Shear-Wave Elastography Imaging Biomarker to Predict Anti-PD-1 Efficacy of Triple-Negative Breast Cancer | ||
Invest Ophthalmol Vis Sci KGF-2 Alleviates Dry Eye Disease by Regulating the HMGB1/TLR4 Pathway | ||
Life (Basel) MPP7 as a Novel Biomarker of Esophageal Cancer: MPP7 Knockdown Inhibits Esophageal Cancer Cell Migration and Invasion | ||
Stem Cells Int Human Umbilical Cord-Mesenchymal Stem Cells Combined With Low Dosage Nintedanib Rather Than Using Alone Mitigates Pulmonary Fibrosis in Mice |
Reviews
What customers say
Users report mixed results depending on the application. The antibody performs well for Western Blot at 1:1000 dilution on liver tissue, and shows strong results for Immunofluorescence and IHC at 1:200 dilution on cell lines (HePG2, Hela). However, one user found it did not work well for Western Blotting. Overall, the antibody is considered reliable for IF and IHC, while WB performance appears somewhat inconsistent across different users and samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-26-2025) | good for wb
|
Kus (Verified Customer) (12-08-2022) | Works good WB but could see working for Immunofluorescence.
|
K (Verified Customer) (02-02-2022) | This Ab works great for immunofluorescence and IHC at 1:200 dilution but this Ab did not work well for western blotting.
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