NUDT6 Polyclonal antibody

• Featured Product
• KD/KO Validated

NUDT6 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11181-1-AP

Synonyms

ASFGF2, FGF 2, FGF AS, FGF2AS, gfg, gfg 1, Nudix motif 6, NUDT6, Protein GFG

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Validation Data Gallery

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  WB analysis of Jurkat using 11181-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11181-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11181-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11181-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11181-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11181-1-AP

  IP Result of anti-NUDT6 (IP:11181-1-AP, 4ug; Detection:11181-1-AP 1:500) with HepG2 cells lysate 4000ug.

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  IHC staining of human gliomas using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11181-1-AP (NUDT6 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11181-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11181-1-AP (NUDT6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

11181-1-AP targets NUDT6 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT6 fusion protein Ag1638
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 6
• Calculated molecular weight: 36 kDa
• Observed molecular weight: 72 kDa
• GenBank accession number: BC009842
• Gene symbol: NUDT6
• Gene ID (NCBI): 11162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.

Publications

Species	Application	Title
human	WB	Mol Endocrinol; Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.; Authors - MacFarlane Leigh-Ann LA
human	WB	Mol Carcinog; Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression.; Authors - MacFarlane Leigh-Ann LA
mouse	WB	Cancer Sci; PMID: 29532562; Authors - Ji-Chang Wang
rat	WB	Genes (Basel); Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.; Authors - Mark Baguma-Nibasheka ; KD Validated
human	WB	Wound Repair Regen; GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.; Authors - Xinying Wang
human	IHC	Burns; Study of the use of recombinant human granulocyte-macrophage colony-stimulating factor hydrogel externally to treat residual wounds of extensive deep partial-thickness burn.; Authors - Lin Yuan