NUDT6 Polyclonal antibody

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  WB analysis of Jurkat using 11181-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11181-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11181-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11181-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11181-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11181-1-AP

  IP result of anti-NUDT6 (IP:11181-1-AP, 4ug; Detection:11181-1-AP 1:500) with HepG2 cells lysate 4000ug.

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  IHC staining of mouse brain using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11181-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUDT6 antibody (11181-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

NUDT6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11181-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.-

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, mouse brain tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11181-1-AP targets NUDT6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT6 fusion protein Ag1638
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 6
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC009842
• Gene Symbol: NUDT6
• Gene ID (NCBI): 11162
• RRID: AB_2236190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53370
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.

Publications

Species	Application	Title
rat	WB,IF	J Physiol; FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling; Authors - Burak Uzay
human	WB	Mol Endocrinol; Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.; Authors - MacFarlane Leigh-Ann LA
human	WB	Mol Carcinog; Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression.; Authors - MacFarlane Leigh-Ann LA
mouse	WB	Cancer Sci; PMID: 29532562; Authors - Ji-Chang Wang
rat	WB	Genes (Basel); Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.; Authors - Mark Baguma-Nibasheka ; KD Validated
human	WB	Wound Repair Regen; GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.; Authors - Xinying Wang