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NUDT6 Polyclonal antibody
NUDT6 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11181-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
Product Information
11181-1-AP targets NUDT6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NUDT6 fusion protein Ag1638 |
Full Name | nudix (nucleoside diphosphate linked moiety X)-type motif 6 |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 72 kDa |
GenBank accession number | BC009842 |
Gene symbol | NUDT6 |
Gene ID (NCBI) | 11162 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.
Protocols
Product Specific Protocols | |
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WB protocol for NUDT6 antibody 11181-1-AP | Download protocol |
IHC protocol for NUDT6 antibody 11181-1-AP | Download protocol |
IF protocol for NUDT6 antibody 11181-1-AP | Download protocol |
IP protocol for NUDT6 antibody 11181-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Endocrinol Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2. | ||
Mol Carcinog Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression. | ||
Genes (Basel) Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.
| ||
Wound Repair Regen GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis. | ||
Burns Study of the use of recombinant human granulocyte-macrophage colony-stimulating factor hydrogel externally to treat residual wounds of extensive deep partial-thickness burn. |