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  • KD/KO Validated

NUDT6 Polyclonal antibody

NUDT6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11181-1-AP

Synonyms

gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.-



Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11181-1-AP targets NUDT6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDT6 fusion protein Ag1638
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 6
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC009842
Gene Symbol NUDT6
Gene ID (NCBI) 11162
RRIDAB_2236190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.

Protocols

Product Specific Protocols
WB protocol for NUDT6 antibody 11181-1-APDownload protocol
IHC protocol for NUDT6 antibody 11181-1-APDownload protocol
IF protocol for NUDT6 antibody 11181-1-APDownload protocol
IP protocol for NUDT6 antibody 11181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

J Physiol

FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling

Authors - Burak Uzay
humanWB

Mol Endocrinol

Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.

Authors - MacFarlane Leigh-Ann LA
humanWB

Mol Carcinog

Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression.

Authors - MacFarlane Leigh-Ann LA
mouseWB

Cancer Sci

PMID: 29532562

Authors - Ji-Chang Wang
ratWB

Genes (Basel)

Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.

Authors - Mark Baguma-Nibasheka
  • KD Validated
humanWB

Wound Repair Regen

GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.

Authors - Xinying Wang