NUDT6 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• NUDT6 Polyclonal antibody (11181-1-AP)

• Featured Product
• KD/KO Validated

NUDT6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11181-1-AP Copied!

Publications (8)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.-

• 

  WB analysis of Jurkat using 11181-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 11181-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat liver using 11181-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat heart using 11181-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 11181-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 11181-1-AP

  IP result of anti-NUDT6 (IP:11181-1-AP, 4ug; Detection:11181-1-AP 1:500) with HepG2 cells lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 11181-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUDT6 antibody (11181-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for NUDT6 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, mouse brain tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11181-1-AP targets NUDT6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1638

  Product name: Recombinant human NUDT6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-316 aa of BC009842

  Sequence: MRQPLSWGRWRAMLARTYGPGPSAGYRWASGAQGYVRNPPVGACDLQGELDRFGGISVRLARLDALDRLDAAAFQKGLQAAVQQWRSEGRTAVWLHIPILQSRFIAPAASLGFCFHHAESDSSTLTLWLREGPSRLPGYASHQVGVAGAVFDESTRKILVVQDRNKLKNMWKFPGGLSEPEEDIGDTAVREVFEETGIKSEFRSVLSIRQQHTNPGAFGKSDMYIICRLKPYSFTINFCQEECLRCEWMDLNDLAKTENTTPITSRVARLLLYGYREGFDKIDLTVEELPAVYTGLFYKLYHKELPENYKTMKGID

• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 6
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC009842
• Gene Symbol: NUDT6
• Gene ID (NCBI): 11162
• RRID: AB_2236190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53370
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.

Publications

What published studies show

The anti-FGF2 antibody (Cat# 11181-1-AP) has 8 citations published in journals including Frontiers in Immunology, Cancer Science, Molecular Endocrinology, The Journal of Physiology, Wound Repair and Regeneration, Molecular Carcinogenesis, Genes (Basel), and Burns. Research fields span endometriosis pain, breast tumor angiogenesis, FGF2 antisense RNA regulation, depression and neurogenesis, wound healing, cell adhesion and cell-cycle progression, and burn treatment. Applications include WB and IF.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Front Immunol; GPR30-mediated non-classic estrogen pathway in mast cells participates in endometriosis pain via the production of FGF2; Authors - Xinxin Xu
mouse	WB	Cancer Sci; PMID: 29532562; Authors - Ji-Chang Wang
human	WB	Mol Endocrinol; Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.; Authors - MacFarlane Leigh-Ann LA
rat	WB,IF	J Physiol; FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling; Authors - Burak Uzay
human	WB	Wound Repair Regen; GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.; Authors - Xinying Wang
human	WB	Mol Carcinog; Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression.; Authors - MacFarlane Leigh-Ann LA