Validation Data Gallery
|Positive WB detected in||Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11181-1-AP targets NUDT6 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NUDT6 fusion protein Ag1638|
|Full Name||nudix (nucleoside diphosphate linked moiety X)-type motif 6|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||72 kDa|
|GenBank accession number||BC009842|
|Gene ID (NCBI)||11162|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.
|Product Specific Protocols|
|WB protocol for NUDT6 antibody 11181-1-AP||Download protocol|
|IHC protocol for NUDT6 antibody 11181-1-AP||Download protocol|
|IF protocol for NUDT6 antibody 11181-1-AP||Download protocol|
|IP protocol for NUDT6 antibody 11181-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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