Various lysates were subjected to SDS PAGE followed by western blot with 18592-1-AP (FOXO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 18592-1-AP( FOXO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

L02 cells were subjected to SDS PAGE followed by western blot with 18592-1-AP( FOXO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 18592-1-AP( FOXO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 18592-1-AP( FOXO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 18592-1-AP( FOXO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 18592-1-AP( FOXO1 Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 18592-1-AP( FOXO1 Antibody) at dilution of 1:200 (under 40x lens)

1X10^6 HeLa cells were stained with 0.2ug FOXO1 antibody (18592-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.