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FOXO1 Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 18592-1-AP

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Con: 27 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, chIP, ELISA
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Images See all

FOXO1 Antibody WB HeLa cells 18592-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 18592-1-AP (FOXO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

FOXO1 Antibody IHC mouse brain tissue 18592-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18592-1-AP( FOXO1 Antibody) at dilution of 1:200 (under 40x lens)

FOXO1 Antibody FC HeLa cells 18592-1-AP

1X10^6 HeLa cells were stained with 0.2ug FOXO1 antibody (18592-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
HeLa cells, mouse liver tissue, L02 cells, mouse brain tissue
Positive IHC detected in:
mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:3000
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
FOXO1 fusion protein Ag13296
Full name:
forkhead box O1
Calculated molecular weight:
655aa,70 kDa
Observed molecular weight:
70-80 kDa
GenBank accession number:
BC021981
Gene ID (NCBI):
2308
Gene symbol
FOXO1
Synonyms
FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A

Background

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).


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