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FOXO3A Antibody 1 Publications

Mouse Monoclonal| Catalog number: 66428-1-Ig |CloneNo.: 1F12D11

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

FOXO3A Antibody WB HEK-293 cells 66428-1-Ig

WB result of FOXO3A antibody (66428-1-Ig, 1:12,000) with si-Control and si-FOXO3A transfected HEK293 cells.

FOXO3A Antibody WB MCF-7 cells 66428-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

FOXO3A Antibody IP HEK-293 cells 66428-1-Ig

IP result of anti-FOXO3A(IP:66428-1-Ig, 4ug; Detection:66428-1-Ig 1:1000) with HEK-293 cells lysate 2200 ug

FOXO3A Antibody IHC human breast cancer tissue 66428-1-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

FOXO3A Antibody WB HEK-293 cells 66428-1-Ig

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66428-1-Ig( FOXO3A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

FOXO3A Antibody IF HepG2 cells 66428-1-Ig

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 66428-1-Ig(FOXO3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-mouse IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human breast cancer tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:250-1:1000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
FOXO3A fusion protein Ag1289
Full name:
forkhead box O3
Calculated molecular weight:
673aa,71 kDa
Observed molecular weight:
85-90 kDa
GenBank accession number:
BC021224
Gene ID (NCBI):
2309
Gene symbol
FOXO3
Synonyms
AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A

Background

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa，but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after insulin stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)


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