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FOXO3A Monoclonal antibody

FOXO3A Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1F12D11

Cat no : 66428-1-Ig

Synonyms

AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A

Validation Data Gallery

  • WB analysis using 66428-1-Ig

    WB analysis using 66428-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66428-1-Ig

    WB analysis of HEK-293 using 66428-1-Ig

    WB result of FOXO3A antibody (66428-1-Ig, 1:12,000) with si-Control and si-FOXO3A transfected HEK293 cells.

  • IP experiment of HEK-293 using 66428-1-Ig

    IP experiment of HEK-293 using 66428-1-Ig

    IP result of anti-FOXO3A (IP:66428-1-Ig, 4ug; Detection:66428-1-Ig 1:1000) with HEK-293 cells lysate 2200 ug.

  • IHC staining of human breast cancer using 66428-1-Ig

    IHC staining of human breast cancer using 66428-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 66428-1-Ig

    WB analysis of HEK-293 using 66428-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66428-1-Ig

    IF Staining of HepG2 using 66428-1-Ig

    Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66428-1-Ig(FOXO3A antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66428-1-Ig targets FOXO3A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, macaque, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FOXO3A fusion protein Ag1289
Full Name forkhead box O3
Calculated molecular weight 673 aa, 71 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC021224
Gene symbol FOXO3
Gene ID (NCBI) 2309
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa，but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)

Protocols

Product Specific Protocols
WB protocol for FOXO3A antibody 66428-1-IgDownload protocol
IHC protocol for FOXO3A antibody 66428-1-IgDownload protocol
IF protocol for FOXO3A antibody 66428-1-IgDownload protocol
IP protocol for FOXO3A antibody 66428-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
macaqueIHC

Front Neurosci

Role of FOXO3 Activated by HIV-1 Tat in HIV-Associated Neurocognitive Disorder Neuronal Apoptosis.

Authors - Huaqian Dong
View Article
mouseWB,IF,IP

Cell Death Discov

TGR5 deficiency aggravates hepatic ischemic/reperfusion injury via inhibiting SIRT3/FOXO3/HIF-1ɑ pathway.

Authors - Qi Wang
View Article
mouse

Int J Rheum Dis

Protective effects of 2,3,5,4-tetrahydroxystilbene-2-o-β-D-glucoside against osteoporosis: Current knowledge and proposed mechanisms.

Authors - Jinkang Zhang
View Article
ratWB,IF

Mar Drugs

Fucoxanthin Alleviates Oxidative Stress through Akt/Sirt1/FoxO3α Signaling to Inhibit HG-Induced Renal Fibrosis in GMCs.

Authors - Guanyu Yang
View Article
ratWB

Int Immunopharmacol

Syringin exerts neuroprotective effects in a rat model of cerebral ischemia through the FOXO3a/NF-κB pathway.

Authors - Junyi Tan
View Article
humanWB

J Cell Mol Med

Altered m6 A modification is involved in up-regulated expression of FOXO3 in luteinized granulosa cells of non-obese polycystic ovary syndrome patients.

Authors - Shen Zhang
View Article