FOXP3 Polyclonal antibody

FOXP3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22228-1-AP

Synonyms

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID



Tested Applications

Positive WB detected inHeLa cells, multi-cells, multi-cells/tissue, A375 cells, mouse brain tissue, HEK-293 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22228-1-AP is FOXP3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXP3 fusion protein Ag17571
Full Name forkhead box P3
Calculated molecular weight 431 aa, 47 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC113401
Gene symbol FOXP3
Gene ID (NCBI) 50943
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Protocols

Product Specific Protocols
WB protocol for FOXP3 antibody 22228-1-APDownload protocol
IP protocol for FOXP3 antibody 22228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

Changes among TGF-β1+ Breg cells and helper T cell subsets in a murine model of allergic rhinitis with prolonged OVA challenge.

Authors - Meng Wang
mouseIHC

Am J Cancer Res

SFRP4 is a prognostic marker and correlated with Treg cell infiltration in pancreatic ductal adenocarcinoma.

Authors - Min-Wei Yang
humanIHC

World J Gastroenterol

Clinical significance of programmed death 1/programmed death ligand 1 pathway in gastric neuroendocrine carcinomas.

Authors - Min-Wei Yang
ratWB

Biomed Pharmacother

Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats.

Authors - Yu Long
mouseIF

Cancer Res

Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.

Authors - Binghao Li
humanWB,IHC

J Immunol Res

DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma.

Authors - Jianyu Yang