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GATA2 Monoclonal antibody

GATA2 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, Rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2B5A1

Cat no : 67722-1-Ig

Synonyms

FLJ45948, GATA binding protein 2, GATA2, NFE1B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis using 67722-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of bEnd.3 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of bEnd.3 using 67722-1-Ig

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of NIH/3T3 using 67722-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of PC-12 using 67722-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of K-562 using 67722-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of HEK-293 using 67722-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of HepG2 using 67722-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of HeLa using 67722-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of LNCaP using 67722-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of HUVEC using 67722-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of PC-12 using 67722-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    WB analysis of NIH/3T3 using 67722-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    IF Staining of L02 using 67722-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using GATA2 antibody (67722-1-Ig, Clone: 2B5A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of K-562 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    FC experiment of K-562 using 67722-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human GATA2 (67722-1-Ig, Clone:2B5A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of K-562 cells using GATA2 Monoclonal antibody (67722-1-Ig)

    FC experiment of K-562 using 67722-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human GATA2 (67722-1-Ig, Clone:2B5A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HUVEC cells, LNCaP cells, PC-12 cells, HepG2 cells, HEK-293 cells, K-562 cells, NIH/3T3 cells, bEnd.3 cells
Positive IF detected inL02 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67722-1-Ig targets GATA2 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, Rat samples.

Tested Reactivity Human, mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA2 fusion protein Ag1557
Full Name GATA binding protein 2
Observed Molecular Weight50 kDa
GenBank Accession NumberBC018988
Gene Symbol GATA2
Gene ID (NCBI) 2624
RRIDAB_2882909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.

Protocols

Product Specific Protocols
WB protocol for GATA2 antibody 67722-1-IgDownload protocol
IF protocol for GATA2 antibody 67722-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols